Detailed information of TA system TA046115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1860763..1861358
• Replicon: chromosome
• Accession: NZ_CP007147
• Organism: Pseudomonas aeruginosa YL84

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: AI22_RS29835
• Protein ID: WP_003113526.1
• Coordinates: 1860763..1861041 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AI22_RS08565
• Protein ID: WP_003113527.1
• Coordinates: 1861053..1861358 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AI22_RS08550	1856188..1857426	+	1239	WP_014603324.1	C69 family dipeptidase	-
AI22_RS08555	1857488..1858135	+	648	WP_003095021.1	carbonate dehydratase	-
AI22_RS08560	1858206..1860434	-	2229	WP_023130306.1	TonB-dependent receptor	-
AI22_RS29835	1860763..1861041	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AI22_RS08565	1861053..1861358	+	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
AI22_RS29840	1861677..1864148	-	2472	WP_079282650.1	SIR2 family protein	-
AI22_RS29845	1864283..1864615	-	333	WP_079282649.1	hypothetical protein	-
AI22_RS08570	1865066..1865338	+	273	WP_025324668.1	hypothetical protein	-
AI22_RS08575	1865331..1865546	+	216	WP_042911940.1	hypothetical protein	-
AI22_RS29855	1865550..1865839	+	290	Protein_1692	DUF5447 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T46115 WP_003113526.1 NZ_CP007147:1860763-1861041 [Pseudomonas aeruginosa YL84]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T46115 NZ_CP007147:1860763-1861041 [Pseudomonas aeruginosa YL84]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT46115 WP_003113527.1 NZ_CP007147:1861053-1861358 [Pseudomonas aeruginosa YL84]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT46115 NZ_CP007147:1861053-1861358 [Pseudomonas aeruginosa YL84]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3