Detailed information of TA system TA046088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3464642..3465369
• Replicon: chromosome
• Accession: NZ_CP007136
• Organism: Escherichia coli O145:H28 str. RM12581

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: ECRM12581_RS17845
• Protein ID: WP_000547563.1
• Coordinates: 3465058..3465369 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECRM12581_RS17840
• Protein ID: WP_000126294.1
• Coordinates: 3464642..3465061 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM12581_RS17820	3459691..3460218	-	528	WP_001078777.1	electron transport protein HydN	-
ECRM12581_RS17825	3460367..3461377	-	1011	WP_032161993.1	DNA-binding transcriptional regulator AscG	-
ECRM12581_RS17830	3461638..3463095	+	1458	WP_001107816.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
ECRM12581_RS17835	3463104..3464528	+	1425	WP_000110310.1	6-phospho-beta-glucosidase AscB	-
ECRM12581_RS17840	3464642..3465061	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
ECRM12581_RS17845	3465058..3465369	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
ECRM12581_RS17850	3465531..3466286	-	756	WP_001400787.1	hypothetical protein	-
ECRM12581_RS17855	3466329..3466796	-	468	WP_000132960.1	hydrogenase maturation peptidase HycI	-
ECRM12581_RS17860	3466789..3467199	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
ECRM12581_RS17865	3467196..3467963	-	768	WP_000067400.1	formate hydrogenlyase subunit HycG	-
ECRM12581_RS17870	3467963..3468505	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
ECRM12581_RS17875	3468515..3470224	-	1710	WP_001288147.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T46088 WP_000547563.1 NZ_CP007136:c3465369-3465058 [Escherichia coli O145:H28 str. RM12581]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T46088 NZ_CP007136:c3465369-3465058 [Escherichia coli O145:H28 str. RM12581]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT46088 WP_000126294.1 NZ_CP007136:c3465061-3464642 [Escherichia coli O145:H28 str. RM12581]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT46088 NZ_CP007136:c3465061-3464642 [Escherichia coli O145:H28 str. RM12581]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACAATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22