Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2617021..2617523 Replicon chromosome
Accession NZ_CP007136
Organism Escherichia coli O145:H28 str. RM12581

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECRM12581_RS13715 Protein ID WP_000767829.1
Coordinates 2617021..2617275 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECRM12581_RS13720 Protein ID WP_001259255.1
Coordinates 2617272..2617523 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM12581_RS13690 2612036..2612263 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
ECRM12581_RS13695 2612277..2613335 - 1059 WP_000492332.1 transport protein YeeE -
ECRM12581_RS13700 2613514..2614872 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECRM12581_RS33670 2614862..2614924 - 63 WP_010723108.1 membrane protein YoeI -
ECRM12581_RS13705 2615139..2616068 - 930 WP_000803351.1 LysR family transcriptional regulator -
ECRM12581_RS13710 2616114..2616938 - 825 WP_000754755.1 SDR family oxidoreductase -
ECRM12581_RS13715 2617021..2617275 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECRM12581_RS13720 2617272..2617523 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECRM12581_RS31110 2617807..2617857 + 51 WP_001364200.1 his operon leader peptide -
ECRM12581_RS13725 2618003..2618902 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECRM12581_RS13730 2618908..2620212 + 1305 WP_001400693.1 histidinol dehydrogenase -
ECRM12581_RS13735 2620209..2621279 + 1071 WP_001099213.1 histidinol-phosphate transaminase -
ECRM12581_RS13740 2621279..2622346 + 1068 WP_000080083.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T46087 WP_000767829.1 NZ_CP007136:c2617275-2617021 [Escherichia coli O145:H28 str. RM12581]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T46087 NZ_CP007136:c2617275-2617021 [Escherichia coli O145:H28 str. RM12581]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT46087 WP_001259255.1 NZ_CP007136:c2617523-2617272 [Escherichia coli O145:H28 str. RM12581]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT46087 NZ_CP007136:c2617523-2617272 [Escherichia coli O145:H28 str. RM12581]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References