Detailed information of TA system TA046074

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1740148..1740519
• Replicon: chromosome
• Accession: NZ_CP007136
• Organism: Escherichia coli O145:H28 str. RM12581

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: ECRM12581_RS08875
• Protein ID: WP_021500490.1
• Coordinates: 1740325..1740519 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1740148..1740326 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM12581_RS08850	1736102..1737475	+	1374	WP_000123735.1	ATP-dependent RNA helicase DbpA	-
ECRM12581_RS08855	1737604..1738539	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
ECRM12581_RS30655	1738592..1739827	-	1236	WP_000040838.1	site-specific integrase	-
ECRM12581_RS08865	1739829..1740044	-	216	WP_000079604.1	excisionase XisR	-
ECRM12581_RS08870	1740144..1740332	-	189	WP_001298826.1	DUF1187 family protein	-
-	1740148..1740326	+	179	NuclAT_0	-	Antitoxin
-	1740148..1740326	+	179	NuclAT_0	-	Antitoxin
-	1740148..1740326	+	179	NuclAT_0	-	Antitoxin
-	1740148..1740326	+	179	NuclAT_0	-	Antitoxin
ECRM12581_RS08875	1740325..1740519	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
ECRM12581_RS08880	1740583..1741635	-	1053	WP_001004423.1	recombinase RecT	-
ECRM12581_RS08885	1741647..1744772	-	3126	WP_025404324.1	exodeoxyribonuclease VIII	-
ECRM12581_RS08890	1744874..1745149	-	276	WP_001519830.1	phage protein	-
ECRM12581_RS08895	1745224..1745400	-	177	WP_000245528.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1738592..1790016	51424

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T46074 WP_021500490.1 NZ_CP007136:c1740519-1740325 [Escherichia coli O145:H28 str. RM12581]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T46074 NZ_CP007136:c1740519-1740325 [Escherichia coli O145:H28 str. RM12581]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT46074 NZ_CP007136:1740148-1740326 [Escherichia coli O145:H28 str. RM12581]
GAGGACTGAAGTTGCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin