Detailed information of TA system TA046066

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 61603..62029
• Replicon: plasmid pO145-12761
• Accession: NZ_CP007135
• Organism: Escherichia coli O145:H28 str. RM12761

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ECRM12761_RS28170
• Protein ID: WP_001312861.1
• Coordinates: 61603..61761 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	61805..62029 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM12761_RS28130	56652..56879	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
ECRM12761_RS28135	56974..57660	-	687	WP_025380718.1	PAS domain-containing protein	-
ECRM12761_RS28140	57851..58234	-	384	WP_000124981.1	relaxosome protein TraM	-
ECRM12761_RS28145	58511..59158	+	648	WP_122994606.1	transglycosylase SLT domain-containing protein	-
ECRM12761_RS28150	59455..60276	-	822	WP_001234426.1	DUF945 domain-containing protein	-
ECRM12761_RS28155	60394..60681	-	288	WP_000107535.1	hypothetical protein	-
ECRM12761_RS28170	61603..61761	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	61805..62029	-	225	NuclAT_0	-	Antitoxin
-	61805..62029	-	225	NuclAT_0	-	Antitoxin
-	61805..62029	-	225	NuclAT_0	-	Antitoxin
-	61805..62029	-	225	NuclAT_0	-	Antitoxin
ECRM12761_RS32820	61841..62029	+	189	WP_001299721.1	hypothetical protein	-
ECRM12761_RS28180	62041..62760	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
ECRM12761_RS28185	62757..63191	-	435	WP_025380720.1	conjugation system SOS inhibitor PsiB	-
ECRM12761_RS30980	63246..63443	-	198	Protein_72	hypothetical protein	-
ECRM12761_RS28190	63471..63704	-	234	WP_025380721.1	DUF905 family protein	-
ECRM12761_RS28195	63767..64306	-	540	WP_025380722.1	single-stranded DNA-binding protein	-
ECRM12761_RS33060	64332..64538	-	207	WP_000547944.1	hypothetical protein	-
ECRM12761_RS28205	65147..65338	-	192	WP_001027500.1	hypothetical protein	-
ECRM12761_RS28210	65335..65799	-	465	WP_044697612.1	DUF1380 family protein	-
ECRM12761_RS28215	65799..66101	-	303	WP_072128865.1	antirestriction protein	-
ECRM12761_RS33065	66642..66959	-	318	Protein_79	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	stcE / exeE / exeG / espP / hlyC / hlyA / hlyB / hlyD	1..98067	98067

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T46066 WP_001312861.1 NZ_CP007135:c61761-61603 [Escherichia coli O145:H28 str. RM12761]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T46066 NZ_CP007135:c61761-61603 [Escherichia coli O145:H28 str. RM12761]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT46066 NZ_CP007135:c62029-61805 [Escherichia coli O145:H28 str. RM12761]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin