Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3814478..3815205 Replicon chromosome
Accession NZ_CP007133
Organism Escherichia coli O145:H28 str. RM12761

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag ECRM12761_RS19380 Protein ID WP_000550189.1
Coordinates 3814891..3815205 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ECRM12761_RS19375 Protein ID WP_000560266.1
Coordinates 3814478..3814894 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM12761_RS19365 3810567..3812111 + 1545 WP_000258466.1 hypothetical protein -
ECRM12761_RS19370 3812415..3814433 + 2019 WP_025380597.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
ECRM12761_RS19375 3814478..3814894 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
ECRM12761_RS19380 3814891..3815205 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
ECRM12761_RS19390 3815490..3816626 - 1137 WP_025380598.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
ECRM12761_RS19395 3816711..3817214 + 504 WP_001295542.1 M48 family metallopeptidase -
ECRM12761_RS19400 3817291..3817983 + 693 WP_000942541.1 vancomycin high temperature exclusion protein -
ECRM12761_RS19405 3818062..3819048 + 987 WP_024221603.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T46058 WP_000550189.1 NZ_CP007133:c3815205-3814891 [Escherichia coli O145:H28 str. RM12761]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T46058 NZ_CP007133:c3815205-3814891 [Escherichia coli O145:H28 str. RM12761]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT46058 WP_000560266.1 NZ_CP007133:c3814894-3814478 [Escherichia coli O145:H28 str. RM12761]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT46058 NZ_CP007133:c3814894-3814478 [Escherichia coli O145:H28 str. RM12761]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATTTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References