Detailed information of TA system TA046057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3753459..3754152
• Replicon: chromosome
• Accession: NZ_CP007133
• Organism: Escherichia coli O145:H28 str. RM12761

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: ECRM12761_RS19080
• Protein ID: WP_000415584.1
• Coordinates: 3753856..3754152 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: ECRM12761_RS19075
• Protein ID: WP_000650107.1
• Coordinates: 3753459..3753854 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM12761_RS19065	3749323..3751581	-	2259	WP_001281878.1	DNA topoisomerase IV subunit A	-
ECRM12761_RS19070	3751719..3753326	-	1608	WP_001400746.1	ABC transporter substrate-binding protein	-
ECRM12761_RS19075	3753459..3753854	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
ECRM12761_RS19080	3753856..3754152	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
ECRM12761_RS19085	3754357..3754839	-	483	WP_000183497.1	GyrI-like domain-containing protein	-
ECRM12761_RS19090	3754892..3755284	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
ECRM12761_RS19095	3755436..3756095	+	660	WP_001221502.1	two-component system response regulator QseB	-
ECRM12761_RS19100	3756092..3757441	+	1350	WP_000673396.1	two-component system sensor histidine kinase QseC	-
ECRM12761_RS19105	3757523..3757819	-	297	WP_000912118.1	DUF2645 family protein	-
ECRM12761_RS19110	3758177..3758758	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
ECRM12761_RS19115	3758789..3759103	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T46057 WP_000415584.1 NZ_CP007133:c3754152-3753856 [Escherichia coli O145:H28 str. RM12761]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T46057 NZ_CP007133:c3754152-3753856 [Escherichia coli O145:H28 str. RM12761]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGACTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT46057 WP_000650107.1 NZ_CP007133:c3753854-3753459 [Escherichia coli O145:H28 str. RM12761]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT46057 NZ_CP007133:c3753854-3753459 [Escherichia coli O145:H28 str. RM12761]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACACAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65