Detailed information of TA system TA046054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3392254..3392981
• Replicon: chromosome
• Accession: NZ_CP007133
• Organism: Escherichia coli O145:H28 str. RM12761

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: ECRM12761_RS17350
• Protein ID: WP_000547563.1
• Coordinates: 3392670..3392981 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECRM12761_RS17345
• Protein ID: WP_000126294.1
• Coordinates: 3392254..3392673 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM12761_RS17325	3387303..3387830	-	528	WP_001078777.1	electron transport protein HydN	-
ECRM12761_RS17330	3387979..3388989	-	1011	WP_032161993.1	DNA-binding transcriptional regulator AscG	-
ECRM12761_RS17335	3389250..3390707	+	1458	WP_001107816.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
ECRM12761_RS17340	3390716..3392140	+	1425	WP_025380565.1	6-phospho-beta-glucosidase AscB	-
ECRM12761_RS17345	3392254..3392673	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
ECRM12761_RS17350	3392670..3392981	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
ECRM12761_RS17355	3393143..3393898	-	756	WP_001400787.1	hypothetical protein	-
ECRM12761_RS17360	3393941..3394408	-	468	WP_000132960.1	hydrogenase maturation peptidase HycI	-
ECRM12761_RS17365	3394401..3394811	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
ECRM12761_RS17370	3394808..3395575	-	768	WP_000067400.1	formate hydrogenlyase subunit HycG	-
ECRM12761_RS17375	3395575..3396117	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
ECRM12761_RS17380	3396127..3397836	-	1710	WP_001288147.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T46054 WP_000547563.1 NZ_CP007133:c3392981-3392670 [Escherichia coli O145:H28 str. RM12761]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T46054 NZ_CP007133:c3392981-3392670 [Escherichia coli O145:H28 str. RM12761]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT46054 WP_000126294.1 NZ_CP007133:c3392673-3392254 [Escherichia coli O145:H28 str. RM12761]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT46054 NZ_CP007133:c3392673-3392254 [Escherichia coli O145:H28 str. RM12761]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACAATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22