Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2544702..2545204 Replicon chromosome
Accession NZ_CP007133
Organism Escherichia coli O145:H28 str. RM12761

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECRM12761_RS13155 Protein ID WP_000767829.1
Coordinates 2544702..2544956 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECRM12761_RS13160 Protein ID WP_001259255.1
Coordinates 2544953..2545204 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM12761_RS13130 2539717..2539944 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
ECRM12761_RS13135 2539958..2541016 - 1059 WP_000492332.1 transport protein YeeE -
ECRM12761_RS13140 2541195..2542553 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECRM12761_RS32315 2542543..2542605 - 63 WP_010723108.1 membrane protein YoeI -
ECRM12761_RS13145 2542820..2543749 - 930 WP_000803351.1 LysR family transcriptional regulator -
ECRM12761_RS13150 2543795..2544619 - 825 WP_000754755.1 SDR family oxidoreductase -
ECRM12761_RS13155 2544702..2544956 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECRM12761_RS13160 2544953..2545204 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECRM12761_RS29785 2545488..2545538 + 51 WP_001364200.1 his operon leader peptide -
ECRM12761_RS13165 2545684..2546583 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECRM12761_RS13170 2546589..2547893 + 1305 WP_001400693.1 histidinol dehydrogenase -
ECRM12761_RS13175 2547890..2548960 + 1071 WP_001099213.1 histidinol-phosphate transaminase -
ECRM12761_RS13180 2548960..2550027 + 1068 WP_000080083.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T46053 WP_000767829.1 NZ_CP007133:c2544956-2544702 [Escherichia coli O145:H28 str. RM12761]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T46053 NZ_CP007133:c2544956-2544702 [Escherichia coli O145:H28 str. RM12761]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT46053 WP_001259255.1 NZ_CP007133:c2545204-2544953 [Escherichia coli O145:H28 str. RM12761]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT46053 NZ_CP007133:c2545204-2544953 [Escherichia coli O145:H28 str. RM12761]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References