Detailed information of TA system TA046041

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1949272..1949497
• Replicon: chromosome
• Accession: NZ_CP007133
• Organism: Escherichia coli O145:H28 str. RM12761

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: ECRM12761_RS09865
• Protein ID: WP_000813254.1
• Coordinates: 1949272..1949427 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1949439..1949497 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM12761_RS09835	1945097..1946155	-	1059	WP_000483509.1	site-specific DNA-methyltransferase	-
ECRM12761_RS32220	1946307..1946504	-	198	WP_032324269.1	TrmB family transcriptional regulator	-
ECRM12761_RS09845	1946717..1947406	-	690	WP_001064906.1	antiterminator	-
ECRM12761_RS09850	1947403..1947762	-	360	WP_024221875.1	RusA family crossover junction endodeoxyribonuclease	-
ECRM12761_RS09855	1947775..1948824	-	1050	WP_001265167.1	DUF968 domain-containing protein	-
ECRM12761_RS31355	1948826..1949104	-	279	WP_072128871.1	hypothetical protein	-
ECRM12761_RS09865	1949272..1949427	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	1949439..1949497	+	59	-	-	Antitoxin
ECRM12761_RS31360	1949674..1949778	-	105	WP_001278450.1	hypothetical protein	-
ECRM12761_RS09870	1949894..1950607	-	714	WP_000555106.1	DUF551 domain-containing protein	-
ECRM12761_RS09875	1950808..1951020	-	213	WP_000063625.1	hypothetical protein	-
ECRM12761_RS09880	1951069..1951425	-	357	WP_000403779.1	hypothetical protein	-
ECRM12761_RS31365	1951403..1952121	-	719	Protein_1879	ead/Ea22-like family protein	-
ECRM12761_RS09890	1952118..1952414	-	297	WP_001266130.1	DUF4406 domain-containing protein	-
ECRM12761_RS09895	1952411..1952803	-	393	WP_022582018.1	DUF977 family protein	-
ECRM12761_RS09900	1952819..1953589	-	771	WP_000450861.1	DUF1627 domain-containing protein	-
ECRM12761_RS09905	1953619..1954359	-	741	WP_000790459.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	1897842..1979861	82019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T46041 WP_000813254.1 NZ_CP007133:c1949427-1949272 [Escherichia coli O145:H28 str. RM12761]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T46041 NZ_CP007133:c1949427-1949272 [Escherichia coli O145:H28 str. RM12761]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT46041 NZ_CP007133:1949439-1949497 [Escherichia coli O145:H28 str. RM12761]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTTTGTGAAGCATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin