Detailed information of TA system TA046030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-DinJ
• Location: 1548235..1548799
• Replicon: chromosome
• Accession: NZ_CP007122
• Organism: Lactobacillus paracasei N1115

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: C2FG57
• Locus tag: AF91_RS07430
• Protein ID: WP_003661092.1
• Coordinates: 1548235..1548537 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A8E0I752
• Locus tag: AF91_RS07435
• Protein ID: WP_016365698.1
• Coordinates: 1548530..1548799 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AF91_RS07415	1543918..1544355	+	438	WP_003661085.1	hypothetical protein	-
AF91_RS07420	1544530..1546707	-	2178	WP_016383421.1	RNA-binding transcriptional accessory protein	-
AF91_RS07425	1546943..1547635	-	693	WP_003661089.1	hypothetical protein	-
AF91_RS07430	1548235..1548537	-	303	WP_003661092.1	type II toxin-antitoxin system YafQ family toxin	Toxin
AF91_RS07435	1548530..1548799	-	270	WP_016365698.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AF91_RS07440	1549173..1549826	-	654	WP_003570049.1	GNAT family N-acetyltransferase	-
AF91_RS07445	1549871..1550686	-	816	WP_003661098.1	DUF2399 domain-containing protein	-
AF91_RS15930	1550711..1551334	-	624	WP_079351422.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-97)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (10-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11383.23 Da
• Isoelectric Point: 10.4225

>T46030 WP_003661092.1 NZ_CP007122:c1548537-1548235 [Lactobacillus paracasei N1115]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELHVDGPRGGWLLIYKIK
QQDLILTLVRTGSHHNLLGK

Nucleotide

• Length: 303 bp

>T46030 NZ_CP007122:c1548537-1548235 [Lactobacillus paracasei N1115]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCTGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCTT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTACATGTTGACGGCCCTCGTGGCGGCTGGTTGCTAATCTATAAAATTAAG
CAGCAAGATCTTATTTTAACCCTAGTTAGAACCGGATCTCATCATAACCTTCTGGGTAAATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9996.71 Da
• Isoelectric Point: 7.2026

>AT46030 WP_016365698.1 NZ_CP007122:c1548799-1548530 [Lactobacillus paracasei N1115]
MAATKKETRLNIRIDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Nucleotide

• Length: 270 bp

>AT46030 NZ_CP007122:c1548799-1548530 [Lactobacillus paracasei N1115]
ATGGCAGCCACAAAGAAAGAAACTCGCTTGAATATTCGTATTGATCCTGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACGATGTTCATGACGAAAATGGTGAAGGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACACCT
GATGACATGTGGAGAGACTTGAATGTATAG