Detailed information of TA system TA045998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 709039..709687
• Replicon: chromosome
• Accession: NZ_CP007044
• Organism: Chania multitudinisentens RB-25

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W0L9E1
• Locus tag: Z042_RS03100
• Protein ID: WP_024912358.1
• Coordinates: 709343..709687 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Z042_RS03095
• Protein ID: WP_024912357.1
• Coordinates: 709039..709350 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Z042_RS03065	705190..705906	-	717	WP_024912351.1	5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase YigB	-
Z042_RS03070	705906..706817	-	912	WP_024912352.1	tyrosine recombinase XerC	-
Z042_RS03075	706814..707518	-	705	WP_024912353.1	DUF484 domain-containing protein	-
Z042_RS03080	707515..708339	-	825	WP_024912354.1	diaminopimelate epimerase	-
Z042_RS03085	708414..708593	-	180	WP_024912355.1	lipoprotein	-
Z042_RS03090	708666..708986	+	321	WP_024912356.1	iron donor protein CyaY	-
Z042_RS03095	709039..709350	-	312	WP_024912357.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
Z042_RS03100	709343..709687	-	345	WP_024912358.1	hypothetical protein	Toxin
Z042_RS03105	709791..712346	-	2556	WP_024912359.1	class I adenylate cyclase	-
Z042_RS03110	712674..713615	+	942	WP_024912360.1	hydroxymethylbilane synthase	-
Z042_RS03115	713612..714352	+	741	WP_024912361.1	uroporphyrinogen-III synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-100)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13629.77 Da
• Isoelectric Point: 9.5939

>T45998 WP_024912358.1 NZ_CP007044:c709687-709343 [Chania multitudinisentens RB-25]
MEALFIELPPFERYRAEYLTDDQFRGLQQILLKNPECGDVIQHTGGLRKIRFADLRRQKGKRGGIRVIYYWYLEKSQFLM
FTLYDKNQKDDLTKPQQELLRGMLEQAKKGKLDE

Nucleotide

• Length: 345 bp

>T45998 NZ_CP007044:c709687-709343 [Chania multitudinisentens RB-25]
ATGGAAGCGTTGTTTATTGAACTTCCGCCATTTGAGCGGTACCGAGCAGAATATCTAACCGATGATCAGTTTCGCGGTTT
GCAACAAATATTGTTGAAAAACCCTGAATGTGGCGACGTAATCCAGCATACCGGTGGATTGCGAAAAATTCGCTTTGCTG
ACTTAAGACGACAGAAAGGTAAACGGGGTGGGATTCGAGTGATTTATTATTGGTACCTGGAGAAATCACAATTCCTGATG
TTTACCTTGTATGATAAAAATCAGAAAGATGATTTGACTAAACCACAGCAAGAGTTACTGCGTGGAATGCTCGAACAAGC
GAAGAAGGGGAAACTGGATGAGTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11766.69 Da
• Isoelectric Point: 10.5202

>AT45998 WP_024912357.1 NZ_CP007044:c709350-709039 [Chania multitudinisentens RB-25]
MSKRNIFDELQEGMEAWGAHNAGRKTLRTHRVTAKMPSMTPVELKAVREKLKLSQAVFAQYLHTGVTTYQNWEQGLARPN
KQAVLLIKMIDRYPQTLAELATL

Nucleotide

• Length: 312 bp

>AT45998 NZ_CP007044:c709350-709039 [Chania multitudinisentens RB-25]
ATGAGTAAACGTAACATTTTTGACGAGCTTCAAGAAGGAATGGAAGCGTGGGGGGCGCATAATGCGGGCAGAAAAACGCT
GCGTACTCATAGAGTCACCGCCAAGATGCCATCAATGACCCCGGTAGAACTAAAAGCAGTGCGTGAAAAATTGAAGTTAT
CCCAAGCCGTTTTTGCTCAGTATTTACATACCGGTGTGACCACGTATCAGAACTGGGAACAAGGCTTGGCGAGGCCGAAC
AAACAAGCGGTATTGTTGATCAAAATGATCGACAGATACCCTCAGACGCTTGCAGAACTGGCAACGCTTTAA

Structures

Toxin

• AlphaFold DB: W0L9E1