Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 2046163..2046388 Replicon chromosome
Accession NZ_CP007037
Organism Shigella flexneri G1663

Toxin (Protein)


Gene name hokW Uniprot ID S1ELB5
Locus tag SF2A_RS11440 Protein ID WP_000813254.1
Coordinates 2046163..2046318 (-) Length 52 a.a.

Antitoxin (RNA)


Gene name sokW
Locus tag -
Coordinates 2046330..2046388 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SF2A_RS11385 2041475..2041825 - 351 WP_005049241.1 IS66 family insertion sequence element accessory protein TnpB -
SF2A_RS11390 2041822..2042496 - 675 WP_004967157.1 IS66-like element accessory protein TnpA -
SF2A_RS11395 2042595..2042810 - 216 WP_000839572.1 class II holin family protein -
SF2A_RS11420 2043606..2044294 - 689 Protein_2077 bacteriophage antitermination protein Q -
SF2A_RS11425 2044291..2044656 - 366 WP_000140020.1 RusA family crossover junction endodeoxyribonuclease -
SF2A_RS11430 2044657..2045715 - 1059 WP_001265248.1 DUF968 domain-containing protein -
SF2A_RS27665 2045717..2045995 - 279 WP_011069426.1 hypothetical protein -
SF2A_RS11440 2046163..2046318 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD Toxin
- 2046330..2046388 + 59 - - Antitoxin
SF2A_RS11450 2046970..2047386 - 417 WP_005069274.1 hypothetical protein -
SF2A_RS11455 2047413..2047553 + 141 Protein_2083 DUF4224 domain-containing protein -
SF2A_RS11460 2047553..2048590 + 1038 WP_004974968.1 tyrosine-type recombinase/integrase -
SF2A_RS11470 2048801..2049598 + 798 WP_001325918.1 DgsA anti-repressor MtfA -
SF2A_RS11480 2049936..2051198 + 1263 Protein_2086 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ipaH9.8 2014313..2054506 40193


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(6-47)


Sequences

Toxin        


Download         Length: 52 a.a.        Molecular weight: 5736.89 Da        Isoelectric Point: 6.1531

>T45984 WP_000813254.1 NZ_CP007037:c2046318-2046163 [Shigella flexneri G1663]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Download         Length: 156 bp

>T45984 NZ_CP007037:c2046318-2046163 [Shigella flexneri G1663]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 59 bp

>AT45984 NZ_CP007037:2046330-2046388 [Shigella flexneri G1663]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TMW0

Antitoxin

Download structure file

References