Detailed information of TA system TA045973

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1381788..1382013
• Replicon: chromosome
• Accession: NZ_CP007037
• Organism: Shigella flexneri G1663

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: SF2A_RS07485
• Protein ID: WP_000813254.1
• Coordinates: 1381858..1382013 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1381788..1381846 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SF2A_RS30755	1377786..1377955	+	170	Protein_1364	hypothetical protein	-
SF2A_RS07445	1378101..1378847	+	747	WP_000788998.1	ATP-binding protein	-
SF2A_RS07450	1378862..1379284	+	423	WP_001118167.1	DUF977 family protein	-
SF2A_RS07455	1379342..1379698	+	357	WP_005048249.1	hypothetical protein	-
SF2A_RS07460	1379791..1380009	+	219	WP_000256998.1	DUF4014 family protein	-
SF2A_RS07465	1380011..1380376	+	366	WP_001229298.1	HNH endonuclease signature motif containing protein	-
SF2A_RS07470	1380373..1381038	+	666	WP_000208062.1	hypothetical protein	-
SF2A_RS07475	1381038..1381403	+	366	WP_000610655.1	DUF551 domain-containing protein	-
-	1381788..1381846	-	59	-	-	Antitoxin
SF2A_RS07485	1381858..1382013	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
SF2A_RS27075	1382519..1383203	-	685	Protein_1373	IS1 family transposase	-
SF2A_RS07505	1383337..1383936	+	600	WP_000940329.1	DUF1367 family protein	-
SF2A_RS07510	1383936..1384226	+	291	WP_000228038.1	DUF1364 domain-containing protein	-
SF2A_RS07515	1384223..1384777	+	555	WP_000640143.1	DUF1133 family protein	-
SF2A_RS07520	1384930..1385103	+	174	WP_000504450.1	hypothetical protein	-
SF2A_RS27085	1385165..1386321	+	1157	WP_094081550.1	IS3-like element IS600 family transposase	-
SF2A_RS07535	1386392..1386874	+	483	WP_000068107.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	1345139..1435284	90145

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T45973 WP_000813254.1 NZ_CP007037:1381858-1382013 [Shigella flexneri G1663]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T45973 NZ_CP007037:1381858-1382013 [Shigella flexneri G1663]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT45973 NZ_CP007037:c1381846-1381788 [Shigella flexneri G1663]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin