Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 407889..408507 Replicon chromosome
Accession NZ_CP007037
Organism Shigella flexneri G1663

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag SF2A_RS02125 Protein ID WP_001291435.1
Coordinates 407889..408107 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag SF2A_RS02130 Protein ID WP_000344797.1
Coordinates 408133..408507 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SF2A_RS02100 (403781) 403781..404092 - 312 WP_000409911.1 MGMT family protein -
SF2A_RS02105 (404471) 404471..404824 + 354 WP_000878140.1 DUF1428 domain-containing protein -
SF2A_RS02110 (404866) 404866..406416 - 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
SF2A_RS02115 (406580) 406580..407050 - 471 WP_000136192.1 YlaC family protein -
SF2A_RS02120 (407166) 407166..407717 - 552 Protein_390 maltose O-acetyltransferase -
SF2A_RS02125 (407889) 407889..408107 - 219 WP_001291435.1 HHA domain-containing protein Toxin
SF2A_RS02130 (408133) 408133..408507 - 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
SF2A_RS02135 (409053) 409053..412202 - 3150 WP_001132484.1 efflux RND transporter permease AcrB -
SF2A_RS02140 (412225) 412225..413418 - 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 402423..403751 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T45972 WP_001291435.1 NZ_CP007037:c408107-407889 [Shigella flexneri G1663]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T45972 NZ_CP007037:c408107-407889 [Shigella flexneri G1663]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT45972 WP_000344797.1 NZ_CP007037:c408507-408133 [Shigella flexneri G1663]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT45972 NZ_CP007037:c408507-408133 [Shigella flexneri G1663]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References