Detailed information of TA system TA045971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 370728..371572
• Replicon: chromosome
• Accession: NZ_CP007037
• Organism: Shigella flexneri G1663

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: SF2A_RS01930
• Protein ID: WP_014532172.1
• Coordinates: 370728..371189 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A5H0K0
• Locus tag: SF2A_RS01940
• Protein ID: WP_005053053.1
• Coordinates: 371261..371572 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SF2A_RS01910 (365896)	365896..367626	-	1731	WP_000645013.1	hypothetical protein	-
SF2A_RS01915 (367679)	367679..369832	-	2154	WP_047343230.1	SEL1-like repeat protein	-
SF2A_RS01925 (369983)	369983..370680	+	698	WP_227804319.1	IS1 family transposase	-
SF2A_RS01930 (370728)	370728..371189	-	462	WP_014532172.1	GNAT family N-acetyltransferase	Toxin
SF2A_RS01940 (371261)	371261..371572	-	312	WP_005053053.1	DUF1778 domain-containing protein	Antitoxin
SF2A_RS01945 (371756)	371756..372646	-	891	WP_000971346.1	heme o synthase	-
SF2A_RS01950 (372658)	372658..372987	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
SF2A_RS01955 (372987)	372987..373601	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
SF2A_RS01960 (373591)	373591..375582	-	1992	Protein_358	cytochrome o ubiquinol oxidase subunit I	-
SF2A_RS01965 (375604)	375604..376101	-	498	Protein_359	COX aromatic rich motif-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	367679..378068	10389
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP037923 Shigella flexneri 5a str. M90T 4168367..4168870 0.009	flank	IS/Tn	-	-	370177..370680	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-133)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-97)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16623.52 Da
• Isoelectric Point: 9.9538

>T45971 WP_014532172.1 NZ_CP007037:c371189-370728 [Shigella flexneri G1663]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 462 bp

>T45971 NZ_CP007037:c371189-370728 [Shigella flexneri G1663]
TTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACTG
TGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGAC
AACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCGG
AAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTACC
AATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11394.07 Da
• Isoelectric Point: 10.3388

>AT45971 WP_005053053.1 NZ_CP007037:c371572-371261 [Shigella flexneri G1663]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT45971 NZ_CP007037:c371572-371261 [Shigella flexneri G1663]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A5H0K0