Detailed information of TA system TA045970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 290859..291553
• Replicon: chromosome
• Accession: NZ_CP007037
• Organism: Shigella flexneri G1663

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: SF2A_RS01475
• Protein ID: WP_001263491.1
• Coordinates: 291155..291553 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: Q0T7Q4
• Locus tag: SF2A_RS01470
• Protein ID: WP_000554759.1
• Coordinates: 290859..291152 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SF2A_RS01445 (285902)	285902..286423	+	522	Protein_268	C40 family peptidase	-
SF2A_RS01450 (286599)	286599..287096	+	498	WP_000006251.1	REP-associated tyrosine transposase RayT	-
SF2A_RS01455 (287212)	287212..288924	-	1713	Protein_270	flagellar biosynthesis protein FlhA	-
SF2A_RS01460 (288896)	288896..289681	+	786	WP_000207560.1	putative lateral flagellar export/assembly protein LafU	-
SF2A_RS01465 (289752)	289752..290807	+	1056	WP_001226172.1	DNA polymerase IV	-
SF2A_RS01470 (290859)	290859..291152	+	294	WP_000554759.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
SF2A_RS01475 (291155)	291155..291553	+	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
SF2A_RS01480 (291563)	291563..292015	+	453	WP_001059860.1	GNAT family N-acetyltransferase	-
SF2A_RS01485 (292261)	292261..293304	+	1044	WP_005060486.1	RNA ligase RtcB family protein	-
SF2A_RS01490 (293420)	293420..293980	+	561	Protein_277	peptide chain release factor H	-
SF2A_RS01495 (294037)	294037..295494	-	1458	WP_001293030.1	cytosol nonspecific dipeptidase	-
SF2A_RS01500 (295756)	295756..296214	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gtrA / gtrB / gtrII	284778..325350	40572

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T45970 WP_001263491.1 NZ_CP007037:291155-291553 [Shigella flexneri G1663]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T45970 NZ_CP007037:291155-291553 [Shigella flexneri G1663]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11232.82 Da
• Isoelectric Point: 7.0153

>AT45970 WP_000554759.1 NZ_CP007037:290859-291152 [Shigella flexneri G1663]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT45970 NZ_CP007037:290859-291152 [Shigella flexneri G1663]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A4P7TR46