Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 290859..291553 Replicon chromosome
Accession NZ_CP007037
Organism Shigella flexneri G1663

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag SF2A_RS01475 Protein ID WP_001263491.1
Coordinates 291155..291553 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag SF2A_RS01470 Protein ID WP_000554759.1
Coordinates 290859..291152 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SF2A_RS01445 (285902) 285902..286423 + 522 Protein_268 C40 family peptidase -
SF2A_RS01450 (286599) 286599..287096 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
SF2A_RS01455 (287212) 287212..288924 - 1713 Protein_270 flagellar biosynthesis protein FlhA -
SF2A_RS01460 (288896) 288896..289681 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
SF2A_RS01465 (289752) 289752..290807 + 1056 WP_001226172.1 DNA polymerase IV -
SF2A_RS01470 (290859) 290859..291152 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
SF2A_RS01475 (291155) 291155..291553 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
SF2A_RS01480 (291563) 291563..292015 + 453 WP_001059860.1 GNAT family N-acetyltransferase -
SF2A_RS01485 (292261) 292261..293304 + 1044 WP_005060486.1 RNA ligase RtcB family protein -
SF2A_RS01490 (293420) 293420..293980 + 561 Protein_277 peptide chain release factor H -
SF2A_RS01495 (294037) 294037..295494 - 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
SF2A_RS01500 (295756) 295756..296214 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrA / gtrB / gtrII 284778..325350 40572


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T45970 WP_001263491.1 NZ_CP007037:291155-291553 [Shigella flexneri G1663]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T45970 NZ_CP007037:291155-291553 [Shigella flexneri G1663]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT45970 WP_000554759.1 NZ_CP007037:290859-291152 [Shigella flexneri G1663]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT45970 NZ_CP007037:290859-291152 [Shigella flexneri G1663]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References