Detailed information of TA system TA045948

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 967791..968331
• Replicon: chromosome
• Accession: NZ_CP007029
• Organism: Thioalkalivibrio paradoxus ARh

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: THITH_RS17580
• Protein ID: WP_084222616.1
• Coordinates: 967791..968033 (+)
• Length: 81 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: THITH_RS17585
• Protein ID: WP_006749049.1
• Coordinates: 968044..968331 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THITH_RS17570	963523..964668	+	1146	WP_006749050.1	HNH endonuclease	-
THITH_RS17575	964732..965097	+	366	WP_084222615.1	VOC family protein	-
THITH_RS04455	965308..966984	-	1677	WP_006746001.1	IS66 family transposase	-
THITH_RS04460	967033..967386	-	354	WP_006746000.1	IS66 family insertion sequence element accessory protein TnpB	-
THITH_RS04465	967383..967616	-	234	WP_006749214.1	hypothetical protein	-
THITH_RS17580	967791..968033	+	243	WP_084222616.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
THITH_RS17585	968044..968331	+	288	WP_006749049.1	HigA family addiction module antidote protein	Antitoxin
THITH_RS04470	968634..969128	-	495	WP_198019469.1	transposase	-
THITH_RS17590	969943..970146	+	204	WP_006749046.1	type II toxin-antitoxin system HicB family antitoxin	-
THITH_RS17595	970143..970370	+	228	WP_083499445.1	type II toxin-antitoxin system HicA family toxin	-
THITH_RS04480	970770..971453	-	684	WP_006749045.1	hypothetical protein	-
THITH_RS04485	971461..972387	-	927	WP_006749044.1	aldo/keto reductase	-
THITH_RS04490	972387..972815	-	429	WP_006749043.1	hypothetical protein	-
THITH_RS04495	973009..973245	+	237	WP_006749042.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	957506..986308	28802

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-80)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-66)

Sequences

Toxin

Protein

• Length: 81 a.a.
• Molecular weight: 8874.96 Da
• Isoelectric Point: 7.3166

>T45948 WP_084222616.1 NZ_CP007029:967791-968033 [Thioalkalivibrio paradoxus ARh 1]
ISQDAYTAGIQAAHAKRLRLILGRLNAASEIKDMDLAGLRLHKLSGGRAGTWSVTVSGNWRVTFRFEDGDAEIVNYEDYH

Nucleotide

• Length: 243 bp

>T45948 NZ_CP007029:967791-968033 [Thioalkalivibrio paradoxus ARh 1]
ATTTCGCAGGACGCTTACACCGCAGGGATACAGGCTGCTCATGCGAAGAGGCTTCGATTGATTCTCGGCAGGCTAAATGC
AGCGTCAGAAATCAAAGACATGGACTTGGCAGGCCTTCGGCTGCACAAGCTCTCTGGGGGTCGCGCCGGGACTTGGTCGG
TAACAGTTAGCGGTAACTGGCGAGTGACCTTCCGGTTCGAGGACGGAGACGCCGAGATAGTGAACTATGAAGATTACCAC
TGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10478.13 Da
• Isoelectric Point: 7.3594

>AT45948 WP_006749049.1 NZ_CP007029:968044-968331 [Thioalkalivibrio paradoxus ARh 1]
MLMHNPPHPGEVLRELCLEPMGLSVTAAAEALGVSRKTLSSVLNGKAGISPEMAIRLSIAFDTSAESWLNQQSQYELWHA
EQHRKELKVKKLVTA

Nucleotide

• Length: 288 bp

>AT45948 NZ_CP007029:968044-968331 [Thioalkalivibrio paradoxus ARh 1]
ATGTTGATGCACAATCCCCCCCATCCTGGTGAAGTGTTGCGTGAGCTTTGTCTGGAGCCCATGGGTCTCTCTGTAACGGC
CGCTGCAGAAGCGCTGGGAGTAAGTCGAAAAACCCTGAGTTCCGTATTGAACGGTAAGGCCGGCATTAGTCCAGAGATGG
CCATTCGACTATCCATTGCGTTTGATACTTCGGCAGAGAGTTGGCTGAACCAGCAGTCCCAGTATGAGCTCTGGCATGCT
GAGCAGCACCGTAAAGAGCTCAAGGTTAAAAAGCTTGTCACCGCCTGA