Detailed information of TA system TA045943

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 409618..410251
• Replicon: chromosome
• Accession: NZ_CP007029
• Organism: Thioalkalivibrio paradoxus ARh

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W0DK25
• Locus tag: THITH_RS01870
• Protein ID: WP_006746209.1
• Coordinates: 409961..410251 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: THITH_RS01865
• Protein ID: WP_006746210.1
• Coordinates: 409618..409923 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THITH_RS01840	404943..406013	-	1071	WP_006746215.1	virulence RhuM family protein	-
THITH_RS01845	406010..407569	-	1560	WP_006746214.1	type I restriction-modification system subunit M	-
THITH_RS01850	407566..408135	-	570	WP_006746213.1	restriction endonuclease subunit S	-
THITH_RS17375	408400..408963	+	564	WP_198019456.1	hypothetical protein	-
THITH_RS17380	408948..409286	+	339	WP_084222596.1	hypothetical protein	-
THITH_RS01860	409290..409496	+	207	WP_006746211.1	hypothetical protein	-
THITH_RS01865	409618..409923	-	306	WP_006746210.1	HigA family addiction module antidote protein	Antitoxin
THITH_RS01870	409961..410251	-	291	WP_006746209.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
THITH_RS01875	410673..411001	-	329	Protein_379	putative addiction module antidote protein	-
THITH_RS01880	411005..411577	-	573	WP_006746207.1	hypothetical protein	-
THITH_RS18640	412548..413036	-	489	WP_006746206.1	hypothetical protein	-
THITH_RS01890	413091..414125	-	1035	WP_006746205.1	DUF4382 domain-containing protein	-
THITH_RS01895	414582..414893	+	312	WP_156925477.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-71)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10668.01 Da
• Isoelectric Point: 9.0661

>T45943 WP_006746209.1 NZ_CP007029:c410251-409961 [Thioalkalivibrio paradoxus ARh 1]
VIVSFRGKGTEDIFDGVDSNAARRTLPANLQARGAKVLDRLNSAVSLQSLSLPGLRLEKLKGNRSGQHSIRINDQYRVCF
RWTEAGAEDVEIVDYH

Nucleotide

• Length: 291 bp

>T45943 NZ_CP007029:c410251-409961 [Thioalkalivibrio paradoxus ARh 1]
GTGATCGTTTCTTTCCGCGGCAAGGGTACCGAGGATATTTTCGATGGCGTGGACTCGAATGCGGCGCGGCGAACCCTGCC
AGCGAACCTTCAGGCCCGTGGTGCCAAGGTCTTGGACCGCCTGAACTCCGCCGTGTCCCTGCAGTCCCTGTCGCTTCCCG
GCCTGCGCCTGGAGAAACTGAAGGGCAATCGATCCGGACAGCACTCGATCCGGATCAACGACCAGTACCGCGTGTGCTTT
CGCTGGACCGAGGCCGGGGCGGAAGATGTCGAGATCGTCGACTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11543.39 Da
• Isoelectric Point: 10.7217

>AT45943 WP_006746210.1 NZ_CP007029:c409923-409618 [Thioalkalivibrio paradoxus ARh 1]
MRRLPKNRRPTPPGEVFLEDFLLPLGITQKDAAARLHISVPRMNEIVRGKRAVTPDTALRFAKFTRTEPEFWLNLQQAVD
LWDAMHLAQAALDEIRPAQTA

Nucleotide

• Length: 306 bp

>AT45943 NZ_CP007029:c409923-409618 [Thioalkalivibrio paradoxus ARh 1]
ATGCGCCGTCTGCCGAAGAATCGTCGTCCGACCCCCCCGGGCGAGGTCTTTCTGGAAGATTTCCTGCTTCCACTCGGGAT
CACACAGAAGGACGCGGCGGCTCGCCTTCACATTTCCGTTCCGCGCATGAACGAGATTGTCAGGGGTAAGCGCGCCGTGA
CCCCCGATACCGCGCTCCGCTTCGCGAAGTTCACGCGGACCGAGCCCGAATTCTGGCTCAACCTTCAACAGGCGGTGGAC
CTGTGGGATGCGATGCACTTGGCCCAGGCTGCGCTGGACGAAATCCGGCCTGCTCAAACGGCCTGA

Structures

Toxin

• AlphaFold DB: W0DK25