Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2266194..2267113 Replicon chromosome
Accession NZ_CP007027
Organism Mycobacterium tuberculosis H37RvSiena

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag Y980_RS21375 Protein ID WP_003900449.1
Coordinates 2266508..2267113 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag Y980_RS10635 Protein ID WP_003410124.1
Coordinates 2266194..2266499 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y980_RS10605 2261205..2262461 - 1257 WP_003410095.1 HNH endonuclease -
Y980_RS10610 2262815..2263390 + 576 WP_003410100.1 hypothetical protein -
Y980_RS10615 2263387..2264427 + 1041 WP_003911735.1 ImmA/IrrE family metallo-endopeptidase -
Y980_RS10620 2264669..2265388 + 720 WP_003410108.1 DUF433 domain-containing protein -
Y980_RS10625 2265378..2265794 + 417 WP_003410114.1 hypothetical protein -
Y980_RS10630 2265810..2266109 - 300 WP_003410120.1 hypothetical protein -
Y980_RS10635 2266194..2266499 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
Y980_RS21375 2266508..2267113 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
Y980_RS10645 2267138..2267497 - 360 WP_003410131.1 hypothetical protein -
Y980_RS10650 2267657..2268115 - 459 Protein_2092 hypothetical protein -
Y980_RS10655 2268112..2269629 - 1518 Protein_2093 DEAD/DEAH box helicase -
Y980_RS10660 2270139..2271137 - 999 WP_003410146.1 cation diffusion facilitator family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T45916 WP_003900449.1 NZ_CP007027:c2267113-2266508 [Mycobacterium tuberculosis H37RvSiena]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T45916 NZ_CP007027:c2267113-2266508 [Mycobacterium tuberculosis H37RvSiena]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT45916 WP_003410124.1 NZ_CP007027:c2266499-2266194 [Mycobacterium tuberculosis H37RvSiena]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT45916 NZ_CP007027:c2266499-2266194 [Mycobacterium tuberculosis H37RvSiena]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCATTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References