Detailed information of TA system TA045869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3452297..3453024
• Replicon: chromosome
• Accession: NZ_CP007025
• Organism: Escherichia albertii KF1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: EAKF1_RS17170
• Protein ID: WP_000547563.1
• Coordinates: 3452297..3452608 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EAKF1_RS17175
• Protein ID: WP_000126297.1
• Coordinates: 3452605..3453024 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAKF1_RS17140	3447439..3449148	+	1710	WP_025238503.1	formate hydrogenlyase subunit HycE	-
EAKF1_RS17145	3449158..3449700	+	543	WP_000493797.1	formate hydrogenlyase subunit HycF	-
EAKF1_RS17150	3449700..3450467	+	768	WP_025238504.1	formate hydrogenlyase subunit HycG	-
EAKF1_RS17155	3450464..3450874	+	411	WP_001291913.1	formate hydrogenlyase assembly protein HycH	-
EAKF1_RS17160	3450867..3451337	+	471	WP_000132954.1	hydrogenase maturation peptidase HycI	-
EAKF1_RS17165	3451380..3452135	+	756	WP_025238505.1	hypothetical protein	-
EAKF1_RS17170	3452297..3452608	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
EAKF1_RS17175	3452605..3453024	+	420	WP_000126297.1	helix-turn-helix domain-containing protein	Antitoxin
EAKF1_RS17180	3453116..3453544	-	429	WP_000536065.1	DUF4259 domain-containing protein	-
EAKF1_RS17190	3453967..3454494	+	528	WP_001078780.1	electron transport protein HydN	-
EAKF1_RS17195	3454647..3456899	+	2253	WP_025238507.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T45869 WP_000547563.1 NZ_CP007025:3452297-3452608 [Escherichia albertii KF1]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T45869 NZ_CP007025:3452297-3452608 [Escherichia albertii KF1]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15446.45 Da
• Isoelectric Point: 4.4596

>AT45869 WP_000126297.1 NZ_CP007025:3452605-3453024 [Escherichia albertii KF1]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT45869 NZ_CP007025:3452605-3453024 [Escherichia albertii KF1]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22