Detailed information of TA system TA045865

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 2611524..2612236
• Replicon: chromosome
• Accession: NZ_CP007025
• Organism: Escherichia albertii KF1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U8WCQ7
• Locus tag: EAKF1_RS12940
• Protein ID: WP_000162413.1
• Coordinates: 2611934..2612236 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EAKF1_RS12935
• Protein ID: WP_000806446.1
• Coordinates: 2611524..2611862 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAKF1_RS12915	2606564..2608547	+	1984	Protein_2452	TonB-dependent heme/hemoglobin receptor ChuA/ShuA	-
EAKF1_RS12920	2608596..2609624	+	1029	WP_001110408.1	hematinate-forming heme oxygenase ChuS	-
EAKF1_RS12925	2609696..2610225	-	530	Protein_2454	helix-turn-helix transcriptional regulator	-
EAKF1_RS12930	2610372..2610938	-	567	WP_001044206.1	outer membrane lipoprotein Slp	-
EAKF1_RS23645	2611287..2611467	-	181	Protein_2456	hypothetical protein	-
EAKF1_RS12935	2611524..2611862	-	339	WP_000806446.1	HigA family addiction module antidote protein	Antitoxin
EAKF1_RS12940	2611934..2612236	-	303	WP_000162413.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EAKF1_RS12945	2612400..2612879	+	480	WP_025238141.1	hypothetical protein	-
EAKF1_RS26640	2612969..2613142	-	174	WP_000553433.1	hypothetical protein	-
EAKF1_RS12955	2613170..2613253	+	84	WP_001295215.1	hypothetical protein	-
EAKF1_RS12960	2613307..2614659	-	1353	WP_025238142.1	glutathione-disulfide reductase	-
EAKF1_RS12965	2614731..2615573	-	843	WP_025238143.1	23S rRNA (adenine(2030)-N(6))-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11871.59 Da
• Isoelectric Point: 9.8664

>T45865 WP_000162413.1 NZ_CP007025:c2612236-2611934 [Escherichia albertii KF1]
MTKKINIKDFRDAWLDDFFEFSTPHKKIPSDIHITLLRKLDIINAATTWKDLRSPPGNRYEELSGKLNGYSSIRVNNQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T45865 NZ_CP007025:c2612236-2611934 [Escherichia albertii KF1]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACACAAAAA
GATACCTTCTGATATTCATATTACATTGTTGCGGAAGCTGGATATTATCAATGCCGCAACTACTTGGAAGGACTTGAGAT
CACCACCAGGTAATCGGTATGAGGAATTGTCAGGGAAGCTAAACGGCTATTCATCAATAAGGGTGAATAATCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13037.92 Da
• Isoelectric Point: 5.2075

>AT45865 WP_000806446.1 NZ_CP007025:c2611862-2611524 [Escherichia albertii KF1]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRITTAEEFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT45865 NZ_CP007025:c2611862-2611524 [Escherichia albertii KF1]
ATGAAACAGGCAACCAGAAAACCGACGACTGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTTAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGCAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGACCTGTGG
GAAGTTGAAAATGATATGCGCGTTCAGGAAGAGTTAAGCCGTATCACTACTGCTGAAGAATTTATTTCTCAGCGGAATCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U8WCQ7