Detailed information of TA system TA045857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1214442..1215092
• Replicon: chromosome
• Accession: NZ_CP007025
• Organism: Escherichia albertii KF1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EAKF1_RS06135
• Protein ID: WP_000263532.1
• Coordinates: 1214751..1215092 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A3T5VD61
• Locus tag: EAKF1_RS06130
• Protein ID: WP_025237546.1
• Coordinates: 1214442..1214741 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAKF1_RS06105	1209674..1209985	-	312	WP_000410252.1	hypothetical protein	-
EAKF1_RS06110	1209990..1210382	-	393	WP_000273381.1	flagellar export chaperone FliS	-
EAKF1_RS06115	1210405..1211721	-	1317	WP_000609691.1	flagellar filament capping protein FliD	-
EAKF1_RS06120	1212132..1213046	-	915	WP_000949079.1	lateral flagellin LafA	-
EAKF1_RS06125	1213533..1214384	+	852	WP_025237545.1	winged helix-turn-helix domain-containing protein	-
EAKF1_RS06130	1214442..1214741	-	300	WP_025237546.1	XRE family transcriptional regulator	Antitoxin
EAKF1_RS06135	1214751..1215092	-	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EAKF1_RS06140	1215168..1216145	-	978	WP_025237547.1	hypothetical protein	-
EAKF1_RS06145	1216162..1217091	-	930	WP_025237548.1	flagellar hook-associated protein FlgL	-
EAKF1_RS06150	1217106..1218482	-	1377	WP_025237549.1	flagellar hook-associated protein FlgK	-
EAKF1_RS06155	1218686..1218985	-	300	WP_025237550.1	rod-binding protein	-
EAKF1_RS06160	1218985..1220085	-	1101	WP_025237551.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (8-88)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T45857 WP_000263532.1 NZ_CP007025:c1215092-1214751 [Escherichia albertii KF1]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T45857 NZ_CP007025:c1215092-1214751 [Escherichia albertii KF1]
ATGTGGGACGTCGAGACAACGGAGACTTTCGACAACTGGTTTGATGCCCAGACTGTTGCGCTGAAAGAAGATTTGCTTGC
TGCAATGCTGATTCTTGCAGAGTATGGCCCACAGTTGGGCAGACCCTTTGCGGACACGGTTAATGATTCACAGTTTTCAA
ATATGAAAGAATTACGTGTCCAACATCAGGGAAACCCGGTACGTGCTTTTTTTGCTTTCGATCCTGCGCGCCGTGGCATT
GTATTATGTGCAGGTGATAAAACGGGGTTAAATGAGAAAAAGTTTTATCGCGATATGATCAAGCTCGCTGATTCTGAATA
CCGTAAGCATCTGAAAAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10962.78 Da
• Isoelectric Point: 9.7665

>AT45857 WP_025237546.1 NZ_CP007025:c1214741-1214442 [Escherichia albertii KF1]
MATLKELMAKQSPESQARIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRVAFQI

Nucleotide

• Length: 300 bp

>AT45857 NZ_CP007025:c1214741-1214442 [Escherichia albertii KF1]
ATGGCTACTCTAAAAGAACTGATGGCAAAGCAAAGCCCTGAAAGCCAGGCGCGTATTGCGGCAAAAGTTGAGGAAATGCG
CCAGACCATCGCCTTGCATCAGCTGAGAGAAGAACTCAATATTTCGCAAACGGAAATGGCGGCAGCGATGGGCATTAAGC
AACCGACCCTTGCAAAAATGGAGCAGGCAGATAACGATCCTCGGTTGTCTACGCTAAAACGCTATGTTGCGGCTTTAGGT
GGGGAATTGAGTATCGACGTTAAACTGCCTAATGGTAAGAGAGTTGCATTTCAGATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A3T5VD61