Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 25044..25606 Replicon chromosome
Accession NZ_CP007025
Organism Escherichia albertii KF1

Toxin (Protein)


Gene name higB Uniprot ID A0A7U8ZNU8
Locus tag EAKF1_RS00120 Protein ID WP_000605679.1
Coordinates 25044..25322 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag EAKF1_RS00125 Protein ID WP_000781370.1
Coordinates 25322..25606 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EAKF1_RS00100 21024..21455 - 432 WP_000152289.1 peroxiredoxin OsmC -
EAKF1_RS00105 21722..21859 + 138 WP_000841563.1 stationary-phase-induced ribosome-associated protein -
EAKF1_RS00110 22016..23713 + 1698 WP_000433442.1 malate dehydrogenase -
EAKF1_RS00115 23848..24858 + 1011 WP_000642385.1 alcohol dehydrogenase AdhP -
EAKF1_RS00120 25044..25322 + 279 WP_000605679.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EAKF1_RS00125 25322..25606 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
EAKF1_RS00130 25834..26487 - 654 WP_000045636.1 formate dehydrogenase-N subunit gamma -
EAKF1_RS00135 26480..27364 - 885 WP_025237029.1 formate dehydrogenase N subunit beta -
EAKF1_RS00140 27377..30424 - 3048 WP_077696004.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10533.10 Da        Isoelectric Point: 7.3995

>T45855 WP_000605679.1 NZ_CP007025:25044-25322 [Escherichia albertii KF1]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASSLVDIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T45855 NZ_CP007025:25044-25322 [Escherichia albertii KF1]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCTGCAAGCAGTCTTGTTGATATCGATATGCCCGGTTACCGACTAC
ACCCATTAAGCGGCGATCGCGATGGCATCTGGGCAATATCTGTATCAGGCAACTGGCGAATCACGTTTGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT45855 WP_000781370.1 NZ_CP007025:25322-25606 [Escherichia albertii KF1]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT45855 NZ_CP007025:25322-25606 [Escherichia albertii KF1]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGACTGTTGACCGGAAAAGCGGCTTTGACGCCAGAAATGGCGA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCTGAG
AAAACGGTGGATGTGTCGCGGTTGCGCCGTCTGGTGACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8ZNU8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References