Detailed information of TA system TA045849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4157297..4157934
• Replicon: chromosome
• Accession: NZ_CP007014
• Organism: Pseudomonas syringae CC1557

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W0MUM0
• Locus tag: N018_RS18310
• Protein ID: WP_025390420.1
• Coordinates: 4157297..4157611 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: N018_RS18315
• Protein ID: WP_024644429.1
• Coordinates: 4157611..4157934 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N018_RS18290	4152486..4153820	+	1335	WP_025390419.1	virulence factor family protein	-
N018_RS18295	4153917..4155812	-	1896	WP_024644431.1	potassium transporter Kup	-
N018_RS18305	4156119..4157210	+	1092	WP_024644430.1	catalase family protein	-
N018_RS18310	4157297..4157611	+	315	WP_025390420.1	hypothetical protein	Toxin
N018_RS18315	4157611..4157934	+	324	WP_024644429.1	helix-turn-helix domain-containing protein	Antitoxin
N018_RS18320	4158234..4159577	+	1344	WP_024644428.1	30S ribosomal protein S12 methylthiotransferase RimO	-
N018_RS18325	4159693..4160133	+	441	WP_024644427.1	GNAT family N-acetyltransferase	-
N018_RS18330	4160227..4160937	+	711	WP_025390421.1	rRNA pseudouridine synthase	-
N018_RS18335	4161014..4161475	+	462	WP_024644425.1	DUF1456 family protein	-
N018_RS18340	4161489..4162196	+	708	WP_024644424.1	tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-88)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11932.65 Da
• Isoelectric Point: 9.8752

>T45849 WP_025390420.1 NZ_CP007014:4157297-4157611 [Pseudomonas syringae CC1557]
MIFIETPVFTSDLRDHLDDEAYRALQTYLAEHPESGALIEDTGGLRKIRWAAHGKGKSGGVRVIYYHVSARYQIRMILIY
RKGIQDSLTDKQKAQLRALNNGWK

Nucleotide

• Length: 315 bp

>T45849 NZ_CP007014:4157297-4157611 [Pseudomonas syringae CC1557]
ATGATCTTCATCGAAACTCCCGTATTCACTTCGGACCTCAGAGATCATTTGGATGACGAAGCATATAGGGCTCTACAAAC
CTACCTCGCTGAGCATCCAGAATCTGGAGCTCTTATTGAAGATACTGGGGGCCTGCGAAAAATCCGCTGGGCGGCTCATG
GCAAGGGAAAAAGCGGGGGTGTGCGAGTTATTTATTACCATGTCTCTGCGCGATACCAGATACGCATGATTTTGATCTAT
CGCAAAGGAATTCAGGATTCGCTGACTGACAAACAGAAGGCTCAGCTTCGTGCTCTCAACAACGGGTGGAAATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12209.77 Da
• Isoelectric Point: 6.2408

>AT45849 WP_024644429.1 NZ_CP007014:4157611-4157934 [Pseudomonas syringae CC1557]
MEKNLFDRLVESMTEMDEIDRGERRPSREFHIDALQVKKIRQATGLSQATFAKRIDVAVGTLRNWEQGRREPEGPARALL
RAIHNDPEHVLAALSFNGDELADTKLN

Nucleotide

• Length: 324 bp

>AT45849 NZ_CP007014:4157611-4157934 [Pseudomonas syringae CC1557]
ATGGAAAAAAATCTCTTTGATCGCCTTGTAGAAAGCATGACCGAGATGGACGAGATAGATCGCGGTGAGCGTCGGCCCTC
GCGTGAATTCCATATCGATGCCCTTCAAGTCAAAAAAATCAGACAAGCGACAGGTCTTTCCCAGGCAACCTTTGCCAAGC
GCATTGATGTCGCAGTCGGGACACTTCGTAATTGGGAGCAGGGACGACGCGAACCTGAGGGGCCAGCGCGTGCATTGCTT
CGAGCGATACACAATGATCCCGAGCACGTTCTTGCAGCATTGAGTTTTAACGGGGATGAACTCGCAGACACGAAGCTGAA
CTGA

Structures

Toxin

• AlphaFold DB: W0MUM0