Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 1445170..1445807 | Replicon | chromosome |
| Accession | NZ_CP006957 | ||
| Organism | Mannheimia haemolytica USDA-ARS-USMARC-184 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | - |
| Locus tag | B824_RS07310 | Protein ID | WP_040080900.1 |
| Coordinates | 1445170..1445475 (+) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | B824_RS07315 | Protein ID | WP_040080901.1 |
| Coordinates | 1445487..1445807 (+) | Length | 107 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| B824_RS07270 | 1441425..1441922 | - | 498 | WP_040080897.1 | hypothetical protein | - |
| B824_RS12800 | 1442312..1442545 | - | 234 | WP_162839428.1 | hypothetical protein | - |
| B824_RS07285 | 1442511..1442840 | - | 330 | WP_040080898.1 | DUF2570 family protein | - |
| B824_RS07290 | 1442841..1443437 | - | 597 | WP_020824120.1 | glycoside hydrolase family 19 protein | - |
| B824_RS07295 | 1443452..1443811 | - | 360 | WP_165876904.1 | phage holin, lambda family | - |
| B824_RS07300 | 1443947..1444420 | - | 474 | WP_006251969.1 | hypothetical protein | - |
| B824_RS07305 | 1444410..1444979 | - | 570 | WP_040080899.1 | recombination protein NinG | - |
| B824_RS07310 | 1445170..1445475 | + | 306 | WP_040080900.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| B824_RS07315 | 1445487..1445807 | + | 321 | WP_040080901.1 | HigA family addiction module antidote protein | Antitoxin |
| B824_RS07320 | 1445874..1446332 | + | 459 | WP_040080902.1 | hypothetical protein | - |
| B824_RS07325 | 1446386..1447426 | - | 1041 | WP_040080511.1 | IS481 family transposase | - |
| B824_RS07335 | 1447561..1448022 | - | 462 | WP_020824123.1 | DUF1367 family protein | - |
| B824_RS07340 | 1448043..1448645 | - | 603 | WP_040080903.1 | adenine methyltransferase | - |
| B824_RS07345 | 1448642..1449289 | - | 648 | WP_032844508.1 | bacteriophage replication protein | - |
| B824_RS07350 | 1449289..1449573 | - | 285 | WP_032844512.1 | hypothetical protein | - |
| B824_RS07355 | 1449711..1450062 | - | 352 | Protein_1372 | hypothetical protein | - |
| B824_RS07360 | 1450059..1450244 | - | 186 | WP_052437193.1 | helix-turn-helix domain-containing protein | - |
| B824_RS07365 | 1450356..1450799 | - | 444 | WP_006252074.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 1422815..1464319 | 41504 | |
| - | flank | IS/Tn | - | - | 1446386..1447426 | 1040 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11995.61 Da Isoelectric Point: 6.4692
>T45826 WP_040080900.1 NZ_CP006957:1445170-1445475 [Mannheimia haemolytica USDA-ARS-USMARC-184]
MFNLTEAHFRDEALYRFFQYGEVSRTIPANLTSVLARKLDMINAAEKLNDLRSPPANRLELLEPKQNNVYSIRVNKQYRL
IFKFENNELSDLYLDPHNYDL
MFNLTEAHFRDEALYRFFQYGEVSRTIPANLTSVLARKLDMINAAEKLNDLRSPPANRLELLEPKQNNVYSIRVNKQYRL
IFKFENNELSDLYLDPHNYDL
Download Length: 306 bp
>T45826 NZ_CP006957:1445170-1445475 [Mannheimia haemolytica USDA-ARS-USMARC-184]
ATGTTTAACTTAACCGAAGCCCATTTTAGAGATGAAGCTCTTTATCGGTTCTTCCAATATGGCGAAGTCAGCAGAACAAT
CCCTGCTAATCTCACAAGCGTATTAGCTCGCAAACTGGATATGATTAATGCAGCTGAAAAGCTGAATGACTTACGCAGTC
CGCCTGCTAATCGCTTAGAGTTACTTGAGCCGAAACAGAACAACGTCTATTCAATTCGAGTAAACAAACAGTATCGATTG
ATTTTCAAGTTTGAAAACAATGAACTCTCTGATTTATACCTTGACCCACACAATTACGACCTATAA
ATGTTTAACTTAACCGAAGCCCATTTTAGAGATGAAGCTCTTTATCGGTTCTTCCAATATGGCGAAGTCAGCAGAACAAT
CCCTGCTAATCTCACAAGCGTATTAGCTCGCAAACTGGATATGATTAATGCAGCTGAAAAGCTGAATGACTTACGCAGTC
CGCCTGCTAATCGCTTAGAGTTACTTGAGCCGAAACAGAACAACGTCTATTCAATTCGAGTAAACAAACAGTATCGATTG
ATTTTCAAGTTTGAAAACAATGAACTCTCTGATTTATACCTTGACCCACACAATTACGACCTATAA
Antitoxin
Download Length: 107 a.a. Molecular weight: 12089.80 Da Isoelectric Point: 6.2152
>AT45826 WP_040080901.1 NZ_CP006957:1445487-1445807 [Mannheimia haemolytica USDA-ARS-USMARC-184]
MMQRKPTPVGEILQEEFLAPLNLKIGDLVEILDVHRNTASNLVNNSCRVSPEMAFKLAKAFDTSPEFWLNLQTATDLWEL
RHNNRFQQSLDSVKAVNHWQGVPAFA
MMQRKPTPVGEILQEEFLAPLNLKIGDLVEILDVHRNTASNLVNNSCRVSPEMAFKLAKAFDTSPEFWLNLQTATDLWEL
RHNNRFQQSLDSVKAVNHWQGVPAFA
Download Length: 321 bp
>AT45826 NZ_CP006957:1445487-1445807 [Mannheimia haemolytica USDA-ARS-USMARC-184]
ATGATGCAACGCAAACCGACCCCGGTTGGGGAAATTCTCCAGGAAGAATTTTTAGCGCCACTTAATCTTAAAATCGGCGA
CCTCGTTGAAATTTTAGACGTACACCGCAACACCGCCAGCAACCTTGTGAATAACAGTTGCCGTGTTAGTCCTGAAATGG
CATTTAAATTGGCAAAAGCCTTTGATACCAGCCCTGAATTTTGGCTCAACCTACAAACCGCAACCGATTTATGGGAACTC
CGCCATAACAACCGCTTCCAACAAAGCCTTGATAGCGTAAAAGCCGTAAATCACTGGCAAGGTGTGCCTGCATTTGCTTA
A
ATGATGCAACGCAAACCGACCCCGGTTGGGGAAATTCTCCAGGAAGAATTTTTAGCGCCACTTAATCTTAAAATCGGCGA
CCTCGTTGAAATTTTAGACGTACACCGCAACACCGCCAGCAACCTTGTGAATAACAGTTGCCGTGTTAGTCCTGAAATGG
CATTTAAATTGGCAAAAGCCTTTGATACCAGCCCTGAATTTTGGCTCAACCTACAAACCGCAACCGATTTATGGGAACTC
CGCCATAACAACCGCTTCCAACAAAGCCTTGATAGCGTAAAAGCCGTAAATCACTGGCAAGGTGTGCCTGCATTTGCTTA
A
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|