Detailed information of TA system TA045798

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 1899702..1900351
• Replicon: chromosome
• Accession: NZ_CP006943
• Organism: Mannheimia varigena USDA-ARS-USMARC-1296

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W0QBP2
• Locus tag: X808_RS08985
• Protein ID: WP_025217997.1
• Coordinates: 1899702..1900034 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: W0QEK0
• Locus tag: X808_RS08990
• Protein ID: WP_025217998.1
• Coordinates: 1900043..1900351 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
X808_RS08960	1894733..1895410	-	678	WP_025217992.1	YggS family pyridoxal phosphate-dependent enzyme	-
X808_RS08965	1895480..1896019	+	540	WP_025217993.1	HAD-IIIA family hydrolase	-
X808_RS08970	1896041..1896349	-	309	WP_038643460.1	DUF3144 domain-containing protein	-
X808_RS08975	1896431..1897393	-	963	WP_025217995.1	RNA polymerase sigma factor RpoH	-
X808_RS08980	1898154..1899395	+	1242	WP_025217996.1	tyrosine-type recombinase/integrase	-
X808_RS08985	1899702..1900034	+	333	WP_025217997.1	hypothetical protein	Toxin
X808_RS08990	1900043..1900351	+	309	WP_025217998.1	helix-turn-helix domain-containing protein	Antitoxin
X808_RS08995	1900650..1901597	+	948	WP_025217999.1	hypothetical protein	-
X808_RS09000	1901683..1902522	+	840	WP_025218000.1	damage-inducible protein DnaD	-
X808_RS09005	1902765..1902974	+	210	WP_025218001.1	AlpA family phage regulatory protein	-
X808_RS09010	1903022..1903273	+	252	WP_051410694.1	hypothetical protein	-
X808_RS09015	1903279..1903611	+	333	WP_025218003.1	hypothetical protein	-
X808_RS09020	1903722..1904288	+	567	WP_025218004.1	ash family protein	-
X808_RS09025	1904281..1904541	+	261	WP_025218005.1	hypothetical protein	-
X808_RS09030	1904538..1904741	+	204	WP_025218006.1	hypothetical protein	-
X808_RS09035	1904734..1905081	+	348	WP_025218007.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1898154..1909709	11555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-81)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12863.59 Da
• Isoelectric Point: 6.3935

>T45798 WP_025217997.1 NZ_CP006943:1899702-1900034 [Mannheimia varigena USDA-ARS-USMARC-1296]
MNLTFIELPPFERFRDENLTDEDYRAFQNELLDNPETGDLIQGLNGLRKIRLADSKRNKGKRGGIRAIYYYFISRDQVYL
VTAYGKDKQEDLTTEQRKSLSAIIDLIKTL

Nucleotide

• Length: 333 bp

>T45798 NZ_CP006943:1899702-1900034 [Mannheimia varigena USDA-ARS-USMARC-1296]
ATGAATTTGACCTTTATTGAGTTACCGCCTTTTGAGCGTTTTAGAGATGAAAACCTAACTGATGAGGATTATAGAGCTTT
TCAAAATGAGCTATTAGACAACCCTGAAACAGGAGACTTAATACAAGGCTTGAATGGTCTAAGAAAAATCAGACTGGCAG
ATAGCAAACGCAACAAAGGGAAACGTGGCGGAATAAGAGCTATTTATTACTATTTTATCAGTCGAGACCAAGTTTATTTA
GTTACCGCATACGGCAAAGATAAACAGGAAGATTTGACTACTGAACAACGTAAATCATTGAGTGCTATCATTGATTTAAT
AAAGACTTTATAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11602.34 Da
• Isoelectric Point: 10.0901

>AT45798 WP_025217998.1 NZ_CP006943:1900043-1900351 [Mannheimia varigena USDA-ARS-USMARC-1296]
MATLYDDLLEGLTAMKEHLEGKRTLRTETLSRPEPLHISADEVKAIRARLNLSQAVFAKRLRTSTKTYQGWEQGKSTPNP
QATILLRLVEQSPQVFEQIARL

Nucleotide

• Length: 309 bp

>AT45798 NZ_CP006943:1900043-1900351 [Mannheimia varigena USDA-ARS-USMARC-1296]
ATGGCTACATTATATGATGACTTATTAGAGGGCTTAACGGCAATGAAAGAGCATCTAGAGGGCAAACGAACACTACGCAC
TGAAACACTATCACGCCCTGAGCCGTTACACATTTCAGCTGATGAGGTAAAAGCAATTAGAGCTAGATTAAACCTCTCTC
AAGCGGTATTTGCTAAACGCCTACGCACAAGTACAAAGACCTATCAAGGGTGGGAGCAAGGGAAAAGCACCCCAAACCCA
CAAGCAACTATTCTTTTGCGATTAGTGGAACAATCCCCACAGGTATTTGAACAAATTGCCCGCTTATAA

Structures

Toxin

• AlphaFold DB: W0QBP2

Antitoxin

• AlphaFold DB: W0QEK0