Detailed information of TA system TA045720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3045198..3045811
• Replicon: chromosome
• Accession: NZ_CP006877
• Organism: Rhizobium gallicum bv. gallicum R602

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B4X5E0
• Locus tag: RGR602_RS15005
• Protein ID: WP_039845770.1
• Coordinates: 3045515..3045811 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RGR602_RS15000
• Protein ID: WP_039845769.1
• Coordinates: 3045198..3045506 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RGR602_RS14990	3040264..3040884	-	621	WP_170250802.1	nuclear transport factor 2 family protein	-
RGR602_RS14995	3041240..3044485	+	3246	WP_052451651.1	AAA family ATPase	-
RGR602_RS15000	3045198..3045506	-	309	WP_039845769.1	HigA family addiction module antidote protein	Antitoxin
RGR602_RS15005	3045515..3045811	-	297	WP_039845770.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RGR602_RS15010	3046552..3046743	-	192	WP_022716877.1	DUF3008 family protein	-
RGR602_RS15015	3046858..3047877	-	1020	WP_039845771.1	Gfo/Idh/MocA family oxidoreductase	-
RGR602_RS15020	3048409..3049386	+	978	WP_039845772.1	TRAP transporter substrate-binding protein	-
RGR602_RS15025	3049532..3050110	+	579	WP_039845773.1	TRAP transporter small permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-84)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11572.95 Da
• Isoelectric Point: 6.0795

>T45720 WP_039845770.1 NZ_CP006877:c3045811-3045515 [Rhizobium gallicum bv. gallicum R602]
MIVGFRDEWLRACFVEDVHSRNIPADLESRLFRKLQMIDDATTDQDLRVPPSNYFEKLRGNLEGLHSIHVNRQWRVVFRW
DGSRGEASEVDLDGHSYR

Nucleotide

• Length: 297 bp

>T45720 NZ_CP006877:c3045811-3045515 [Rhizobium gallicum bv. gallicum R602]
ATGATCGTCGGTTTTCGGGACGAATGGTTGCGGGCCTGCTTTGTGGAGGATGTCCACTCCCGGAACATTCCAGCCGACCT
CGAAAGCCGGTTGTTCCGCAAACTCCAAATGATCGACGACGCGACGACCGATCAGGACTTGCGTGTGCCGCCCAGTAACT
ATTTCGAGAAACTACGCGGCAATCTCGAGGGTTTGCATTCAATCCACGTCAACAGACAATGGCGGGTTGTTTTTCGATGG
GATGGCAGTCGTGGCGAGGCGTCCGAGGTCGATCTTGACGGCCATAGCTATCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11541.42 Da
• Isoelectric Point: 10.1840

>AT45720 WP_039845769.1 NZ_CP006877:c3045506-3045198 [Rhizobium gallicum bv. gallicum R602]
MLMTKRKPATVGEILREEFMQPLDLTQAALAEAMGVQRKHVNELCNDRRNVTAATALILARVFGNSPDFWLNVQRRSDLW
DAMHSPKELARIERAKPLVTAA

Nucleotide

• Length: 309 bp

>AT45720 NZ_CP006877:c3045506-3045198 [Rhizobium gallicum bv. gallicum R602]
ATGTTGATGACCAAGCGGAAGCCGGCGACCGTCGGCGAAATCCTGAGGGAAGAATTCATGCAGCCGCTCGACCTGACGCA
GGCGGCCTTGGCCGAGGCAATGGGCGTCCAGCGCAAGCATGTGAACGAATTGTGCAACGACCGGCGTAACGTGACGGCGG
CAACCGCGCTGATCCTGGCGCGCGTGTTTGGTAACAGCCCGGATTTCTGGCTCAACGTGCAGCGGCGCAGCGACTTATGG
GATGCCATGCATAGCCCCAAGGAACTGGCCCGGATCGAGCGCGCGAAGCCGCTAGTGACAGCGGCATAG

Structures

Toxin

• AlphaFold DB: A0A0B4X5E0