Detailed information of TA system TA045706

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4893816..4894418
• Replicon: chromosome
• Accession: NZ_CP006834
• Organism: Escherichia coli APEC O2-211

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: APECO2_RS25545
• Protein ID: WP_000897305.1
• Coordinates: 4894107..4894418 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: APECO2_RS25540
• Protein ID: WP_000356397.1
• Coordinates: 4893816..4894106 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APECO2_RS25520	4890318..4891220	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
APECO2_RS25525	4891217..4891852	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
APECO2_RS25530	4891849..4892778	+	930	WP_039025804.1	formate dehydrogenase accessory protein FdhE	-
APECO2_RS25535	4892993..4893211	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
APECO2_RS25540	4893816..4894106	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
APECO2_RS25545	4894107..4894418	-	312	WP_000897305.1	hypothetical protein	Toxin
APECO2_RS25550	4894647..4895555	+	909	WP_001318161.1	alpha/beta hydrolase	-
APECO2_RS25555	4895619..4896560	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
APECO2_RS25560	4896605..4897042	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
APECO2_RS25565	4897039..4897911	-	873	WP_000920762.1	virulence factor BrkB family protein	-
APECO2_RS25570	4897905..4898504	-	600	WP_001315111.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T45706 WP_000897305.1 NZ_CP006834:c4894418-4894107 [Escherichia coli APEC O2-211]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T45706 NZ_CP006834:c4894418-4894107 [Escherichia coli APEC O2-211]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT45706 WP_000356397.1 NZ_CP006834:c4894106-4893816 [Escherichia coli APEC O2-211]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT45706 NZ_CP006834:c4894106-4893816 [Escherichia coli APEC O2-211]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7