Detailed information of TA system TA045685

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2490240..2490611
• Replicon: chromosome
• Accession: NZ_CP006834
• Organism: Escherichia coli APEC O2-211

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: APECO2_RS13180
• Protein ID: WP_001317028.1
• Coordinates: 2490417..2490611 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2490240..2490418 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APECO2_RS13155	2486195..2487568	+	1374	WP_001272254.1	ATP-dependent RNA helicase DbpA	-
APECO2_RS13160	2487697..2488632	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
APECO2_RS13165	2488684..2489919	-	1236	WP_000040852.1	site-specific integrase	-
APECO2_RS13170	2489921..2490136	-	216	WP_000079604.1	excisionase XisR	-
APECO2_RS13175	2490215..2490424	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	2490240..2490418	+	179	NuclAT_0	-	Antitoxin
-	2490240..2490418	+	179	NuclAT_0	-	Antitoxin
-	2490240..2490418	+	179	NuclAT_0	-	Antitoxin
-	2490240..2490418	+	179	NuclAT_0	-	Antitoxin
APECO2_RS13180	2490417..2490611	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
APECO2_RS13185	2490668..2491477	-	810	WP_000166319.1	recombination protein RecT	-
APECO2_RS13190	2491470..2494070	-	2601	WP_039026005.1	exodeoxyribonuclease VIII	-
APECO2_RS13195	2494172..2494447	-	276	WP_000632297.1	protein RacC	-
APECO2_RS13200	2494522..2494692	-	171	WP_001352098.1	conserved protein, Rac prophage	-
APECO2_RS13205	2494692..2494913	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T45685 WP_001317028.1 NZ_CP006834:c2490611-2490417 [Escherichia coli APEC O2-211]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T45685 NZ_CP006834:c2490611-2490417 [Escherichia coli APEC O2-211]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT45685 NZ_CP006834:2490240-2490418 [Escherichia coli APEC O2-211]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin