Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4340939..4341618 Replicon chromosome
Accession NZ_CP006830
Organism Escherichia coli APEC O18

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag APECO18_RS21495 Protein ID WP_000057524.1
Coordinates 4340939..4341241 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag APECO18_RS21500 Protein ID WP_000806442.1
Coordinates 4341277..4341618 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APECO18_RS21470 4336315..4337967 + 1653 WP_000771762.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
APECO18_RS21475 4338005..4338508 - 504 WP_000667000.1 hypothetical protein -
APECO18_RS21480 4338505..4339305 - 801 WP_000439796.1 hypothetical protein -
APECO18_RS21485 4339329..4339808 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
APECO18_RS21490 4340012..4340806 - 795 WP_000365177.1 TraB/GumN family protein -
APECO18_RS21495 4340939..4341241 + 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
APECO18_RS21500 4341277..4341618 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
APECO18_RS21505 4341676..4344180 - 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
APECO18_RS21510 4344442..4345374 + 933 WP_000883052.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4338005..4350219 12214


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T45674 WP_000057524.1 NZ_CP006830:4340939-4341241 [Escherichia coli APEC O18]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T45674 NZ_CP006830:4340939-4341241 [Escherichia coli APEC O18]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT45674 WP_000806442.1 NZ_CP006830:4341277-4341618 [Escherichia coli APEC O18]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT45674 NZ_CP006830:4341277-4341618 [Escherichia coli APEC O18]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References