Detailed information of TA system TA045672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4112010..4112704
• Replicon: chromosome
• Accession: NZ_CP006830
• Organism: Escherichia coli APEC O18

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: APECO18_RS20425
• Protein ID: WP_047340692.1
• Coordinates: 4112306..4112704 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: APECO18_RS20420
• Protein ID: WP_000554758.1
• Coordinates: 4112010..4112303 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APECO18_RS20400	4107069..4107485	+	417	WP_047340689.1	hypothetical protein	-
APECO18_RS26265	4107509..4107712	+	204	WP_073268666.1	hypothetical protein	-
APECO18_RS20405	4107827..4109917	+	2091	WP_047340690.1	tape measure protein	-
APECO18_RS20410	4110074..4110847	+	774	WP_047340691.1	hypothetical protein	-
APECO18_RS20415	4110906..4111958	+	1053	WP_023277943.1	DNA polymerase IV	-
APECO18_RS20420	4112010..4112303	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
APECO18_RS20425	4112306..4112704	+	399	WP_047340692.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
APECO18_RS20430	4112714..4113166	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
APECO18_RS20435	4113356..4114495	+	1140	WP_000521577.1	RNA ligase RtcB family protein	-
APECO18_RS20440	4114492..4115106	+	615	WP_000602123.1	peptide chain release factor H	-
APECO18_RS20445	4115163..4116620	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
APECO18_RS20450	4116881..4117339	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4101481..4113166	11685

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15390.81 Da
• Isoelectric Point: 8.9483

>T45672 WP_047340692.1 NZ_CP006830:4112306-4112704 [Escherichia coli APEC O18]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPNKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T45672 NZ_CP006830:4112306-4112704 [Escherichia coli APEC O18]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTAATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT45672 WP_000554758.1 NZ_CP006830:4112010-4112303 [Escherichia coli APEC O18]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT45672 NZ_CP006830:4112010-4112303 [Escherichia coli APEC O18]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1QAE3