Detailed information of TA system TA045667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3224336..3224938
• Replicon: chromosome
• Accession: NZ_CP006830
• Organism: Escherichia coli APEC O18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: APECO18_RS16155
• Protein ID: WP_000897302.1
• Coordinates: 3224336..3224647 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: APECO18_RS16160
• Protein ID: WP_000356397.1
• Coordinates: 3224648..3224938 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APECO18_RS16130	3220250..3220849	+	600	WP_001314324.1	glucose-1-phosphatase	-
APECO18_RS16135	3220843..3221715	+	873	WP_000920762.1	virulence factor BrkB family protein	-
APECO18_RS16140	3221712..3222149	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
APECO18_RS16145	3222194..3223135	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
APECO18_RS16150	3223199..3224107	-	909	WP_001331553.1	alpha/beta hydrolase	-
APECO18_RS16155	3224336..3224647	+	312	WP_000897302.1	hypothetical protein	Toxin
APECO18_RS16160	3224648..3224938	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
APECO18_RS16170	3225297..3225575	+	279	WP_001296612.1	hypothetical protein	-
APECO18_RS16175	3225971..3226189	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
APECO18_RS16180	3226405..3227334	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
APECO18_RS16185	3227331..3227966	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
APECO18_RS16190	3227963..3228865	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T45667 WP_000897302.1 NZ_CP006830:3224336-3224647 [Escherichia coli APEC O18]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T45667 NZ_CP006830:3224336-3224647 [Escherichia coli APEC O18]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT45667 WP_000356397.1 NZ_CP006830:3224648-3224938 [Escherichia coli APEC O18]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT45667 NZ_CP006830:3224648-3224938 [Escherichia coli APEC O18]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416