Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1095942..1096444 Replicon chromosome
Accession NZ_CP006830
Organism Escherichia coli APEC O18

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag APECO18_RS05695 Protein ID WP_000767822.1
Coordinates 1095942..1096196 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag APECO18_RS05700 Protein ID WP_001259255.1
Coordinates 1096193..1096444 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APECO18_RS05680 1092435..1093793 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
APECO18_RS26790 1093783..1093845 - 63 WP_010723108.1 membrane protein YoeI -
APECO18_RS05685 1094060..1094989 - 930 WP_000803366.1 LysR family transcriptional regulator -
APECO18_RS05690 1095035..1095859 - 825 WP_000754733.1 SDR family oxidoreductase -
APECO18_RS05695 1095942..1096196 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
APECO18_RS05700 1096193..1096444 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
APECO18_RS25450 1096727..1096777 + 51 WP_001364200.1 his operon leader peptide -
APECO18_RS05705 1096923..1097822 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
APECO18_RS05710 1097828..1099132 + 1305 WP_001297912.1 histidinol dehydrogenase -
APECO18_RS05715 1099129..1100199 + 1071 WP_000108995.1 histidinol-phosphate transaminase -
APECO18_RS05720 1100199..1101266 + 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T45664 WP_000767822.1 NZ_CP006830:c1096196-1095942 [Escherichia coli APEC O18]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T45664 NZ_CP006830:c1096196-1095942 [Escherichia coli APEC O18]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT45664 WP_001259255.1 NZ_CP006830:c1096444-1096193 [Escherichia coli APEC O18]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT45664 NZ_CP006830:c1096444-1096193 [Escherichia coli APEC O18]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References