Detailed information of TA system TA045647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 962536..963167
• Replicon: chromosome
• Accession: NZ_CP006829
• Organism: Thalassolituus oleivorans R6-15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W8FVU5
• Locus tag: R615_RS04325
• Protein ID: WP_025264948.1
• Coordinates: 962853..963167 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: R615_RS04320
• Protein ID: WP_025264947.1
• Coordinates: 962536..962826 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R615_RS04310	960019..961500	+	1482	WP_025264945.1	DUF3696 domain-containing protein	-
R615_RS04315	961497..962468	+	972	WP_155990824.1	hypothetical protein	-
R615_RS04320	962536..962826	-	291	WP_025264947.1	helix-turn-helix domain-containing protein	Antitoxin
R615_RS04325	962853..963167	-	315	WP_025264948.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
R615_RS04330	963434..963733	+	300	WP_025264949.1	hypothetical protein	-
R615_RS04335	963720..964076	+	357	WP_015485607.1	IS66 family insertion sequence element accessory protein TnpB	-
R615_RS04340	964100..965710	+	1611	WP_025264950.1	IS66 family transposase	-
R615_RS04345	965725..966276	+	552	WP_155990825.1	Crp/Fnr family transcriptional regulator	-
R615_RS04350	966376..966801	+	426	WP_025264952.1	MAPEG family protein	-
R615_RS04355	967020..967244	+	225	WP_025264953.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-73)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11994.68 Da
• Isoelectric Point: 7.2015

>T45647 WP_025264948.1 NZ_CP006829:c963167-962853 [Thalassolituus oleivorans R6-15]
MLFIETSVFTQTIKELMSDDEYRELQEELTIQPDKGDVIQGTGGIRKIRAKATGRGKRGGSRVIYYWRSAHDQILMLLAY
PKNDQDDLSPDQKKVLKAIVENWH

Nucleotide

• Length: 315 bp

>T45647 NZ_CP006829:c963167-962853 [Thalassolituus oleivorans R6-15]
ATGTTATTCATTGAGACAAGTGTATTTACGCAAACAATTAAAGAACTGATGTCAGACGACGAATACCGTGAGCTACAAGA
AGAACTAACGATACAACCGGATAAAGGTGATGTTATTCAGGGTACAGGTGGTATTCGCAAGATCCGAGCTAAAGCGACGG
GCCGTGGCAAGCGAGGTGGTTCCCGAGTTATCTATTACTGGCGATCAGCGCATGACCAAATATTAATGCTATTAGCCTAC
CCGAAAAATGATCAAGATGACCTCAGCCCTGATCAGAAAAAAGTACTGAAAGCCATTGTCGAGAACTGGCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10643.41 Da
• Isoelectric Point: 10.4776

>AT45647 WP_025264947.1 NZ_CP006829:c962826-962536 [Thalassolituus oleivorans R6-15]
MKDELFNQLMQSVQEADGILKGKQAPARATQIEIPDVKAIREATGLSQRLFANMIGVSLRTLQNWEQNRRTPDGPARALL
LVVKKNPMVVMEALGS

Nucleotide

• Length: 291 bp

>AT45647 NZ_CP006829:c962826-962536 [Thalassolituus oleivorans R6-15]
ATGAAAGACGAACTTTTTAACCAGTTAATGCAAAGCGTGCAAGAAGCCGACGGCATCCTGAAGGGGAAGCAAGCGCCTGC
GCGTGCCACTCAAATTGAGATTCCCGATGTAAAAGCCATTCGTGAGGCAACAGGCCTGAGTCAACGCTTATTCGCTAACA
TGATCGGTGTATCGCTCCGTACGCTACAAAACTGGGAACAAAATCGTAGAACCCCTGATGGGCCAGCGAGAGCTTTATTA
TTGGTAGTGAAGAAAAATCCTATGGTGGTGATGGAGGCGTTGGGGAGTTAA

Structures

Toxin

• AlphaFold DB: W8FVU5