Detailed information of TA system TA045637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 1075966..1076554
• Replicon: chromosome
• Accession: NZ_CP006824
• Organism: Helicobacter pylori oki422

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: K7Y720
• Locus tag: HPOKI422_RS05075
• Protein ID: WP_015086299.1
• Coordinates: 1076273..1076554 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: X2I3W4
• Locus tag: HPOKI422_RS05070
• Protein ID: WP_025276158.1
• Coordinates: 1075966..1076280 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPOKI422_RS05050	1071557..1072213	+	657	WP_025276156.1	ParA family protein	-
HPOKI422_RS05055	1072295..1072579	+	285	WP_000394639.1	hypothetical protein	-
HPOKI422_RS05060	1072611..1073795	+	1185	WP_025276157.1	hypothetical protein	-
HPOKI422_RS05065	1073820..1075707	-	1888	Protein_957	relaxase/mobilization nuclease domain-containing protein	-
HPOKI422_RS05070	1075966..1076280	+	315	WP_025276158.1	hypothetical protein	Antitoxin
HPOKI422_RS05075	1076273..1076554	+	282	WP_015086299.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
HPOKI422_RS08310	1076825..1078554	-	1730	Protein_960	type IV secretory system conjugative DNA transfer family protein	-
HPOKI422_RS05085	1078555..1079112	-	558	WP_025277016.1	hypothetical protein	-
HPOKI422_RS05090	1079113..1079403	-	291	WP_000159826.1	hypothetical protein	-
HPOKI422_RS05095	1079400..1079855	-	456	WP_025276161.1	hypothetical protein	-
HPOKI422_RS05100	1079905..1080792	-	888	WP_025366525.1	Flp pilus assembly complex ATPase component TadA	-
HPOKI422_RS05105	1080792..1081091	-	300	WP_025309899.1	hypothetical protein	-
HPOKI422_RS05110	1081088..1081351	-	264	WP_025276164.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1015276..1089463	74187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10860.83 Da
• Isoelectric Point: 10.0264

>T45637 WP_015086299.1 NZ_CP006824:1076273-1076554 [Helicobacter pylori oki422]
MLKVRTKKDFLKDFNKHILSGRITESDVASVVDCLKKQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T45637 NZ_CP006824:1076273-1076554 [Helicobacter pylori oki422]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATTTTATCTGGGCGTATTACAGAAAGCGA
TGTTGCAAGCGTTGTTGATTGCCTAAAAAAACAAAAACCACTCCAACAAAAATATTGCGACCATGCTTTGAGTGGCAATC
TTAAAGGGCTGAGAGAGTGTCATGTTAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12212.73 Da
• Isoelectric Point: 5.7657

>AT45637 WP_025276158.1 NZ_CP006824:1075966-1076280 [Helicobacter pylori oki422]
MSNIITDYSQYNEKQLHNFLNSIEKQLLKAEGDKNKAIKKIQECELQERMIRQVLAQKHSQEKEPTQSLLNTIVSKDDPE
CDVSFGDFNDFLQIAKQERERRNA

Nucleotide

• Length: 315 bp

>AT45637 NZ_CP006824:1075966-1076280 [Helicobacter pylori oki422]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACTCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATTCAAGAGTGTGAATTGCAAGAACGAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGTCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCGTAATGCTTAA

Structures

Toxin

• AlphaFold DB: K7Y720

Antitoxin

• AlphaFold DB: X2I3W4