Detailed information of TA system TA045632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 1081963..1082551
• Replicon: chromosome
• Accession: NZ_CP006821
• Organism: Helicobacter pylori oki112

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: K7Y720
• Locus tag: HPOKI112_RS05085
• Protein ID: WP_015086299.1
• Coordinates: 1082270..1082551 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: X2I3W4
• Locus tag: HPOKI112_RS05080
• Protein ID: WP_025276158.1
• Coordinates: 1081963..1082277 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPOKI112_RS05060	1077554..1078210	+	657	WP_025276156.1	ParA family protein	-
HPOKI112_RS05065	1078292..1078576	+	285	WP_000394639.1	hypothetical protein	-
HPOKI112_RS05070	1078608..1079792	+	1185	WP_025276157.1	hypothetical protein	-
HPOKI112_RS05075	1079817..1081704	-	1888	Protein_974	relaxase/mobilization nuclease domain-containing protein	-
HPOKI112_RS05080	1081963..1082277	+	315	WP_025276158.1	hypothetical protein	Antitoxin
HPOKI112_RS05085	1082270..1082551	+	282	WP_015086299.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
HPOKI112_RS05090	1082822..1084549	-	1728	WP_025276159.1	type IV secretory system conjugative DNA transfer family protein	-
HPOKI112_RS05095	1084550..1085107	-	558	WP_025277016.1	hypothetical protein	-
HPOKI112_RS05100	1085108..1085398	-	291	WP_000159826.1	hypothetical protein	-
HPOKI112_RS05105	1085395..1085850	-	456	WP_025276161.1	hypothetical protein	-
HPOKI112_RS05110	1085911..1086786	-	876	WP_025309898.1	Flp pilus assembly complex ATPase component TadA	-
HPOKI112_RS05115	1086786..1087085	-	300	WP_025309899.1	hypothetical protein	-
HPOKI112_RS05120	1087082..1087345	-	264	WP_025276164.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1051752..1087688	35936

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10860.83 Da
• Isoelectric Point: 10.0264

>T45632 WP_015086299.1 NZ_CP006821:1082270-1082551 [Helicobacter pylori oki112]
MLKVRTKKDFLKDFNKHILSGRITESDVASVVDCLKKQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T45632 NZ_CP006821:1082270-1082551 [Helicobacter pylori oki112]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATTTTATCTGGGCGTATTACAGAAAGCGA
TGTTGCAAGCGTTGTTGATTGCCTAAAAAAACAAAAACCACTCCAACAAAAATATTGCGACCATGCTTTGAGTGGCAATC
TTAAAGGGCTGAGAGAGTGTCATGTTAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12212.73 Da
• Isoelectric Point: 5.7657

>AT45632 WP_025276158.1 NZ_CP006821:1081963-1082277 [Helicobacter pylori oki112]
MSNIITDYSQYNEKQLHNFLNSIEKQLLKAEGDKNKAIKKIQECELQERMIRQVLAQKHSQEKEPTQSLLNTIVSKDDPE
CDVSFGDFNDFLQIAKQERERRNA

Nucleotide

• Length: 315 bp

>AT45632 NZ_CP006821:1081963-1082277 [Helicobacter pylori oki112]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACTCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATTCAAGAGTGTGAATTGCAAGAACGAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGTCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCGTAATGCTTAA

Structures

Toxin

• AlphaFold DB: K7Y720

Antitoxin

• AlphaFold DB: X2I3W4