Detailed information of TA system TA045629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 1075959..1076547
• Replicon: chromosome
• Accession: NZ_CP006820
• Organism: Helicobacter pylori oki102

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: K7Y720
• Locus tag: HPOKI102_RS05055
• Protein ID: WP_015086299.1
• Coordinates: 1076266..1076547 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: X2I3W4
• Locus tag: HPOKI102_RS05050
• Protein ID: WP_025276158.1
• Coordinates: 1075959..1076273 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPOKI102_RS05030	1071550..1072206	+	657	WP_025276156.1	ParA family protein	-
HPOKI102_RS05035	1072288..1072572	+	285	WP_000394639.1	hypothetical protein	-
HPOKI102_RS05040	1072604..1073788	+	1185	WP_025276157.1	hypothetical protein	-
HPOKI102_RS05045	1073813..1075700	-	1888	Protein_963	relaxase/mobilization nuclease domain-containing protein	-
HPOKI102_RS05050	1075959..1076273	+	315	WP_025276158.1	hypothetical protein	Antitoxin
HPOKI102_RS05055	1076266..1076547	+	282	WP_015086299.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
HPOKI102_RS05060	1076818..1078545	-	1728	WP_025276159.1	type IV secretory system conjugative DNA transfer family protein	-
HPOKI102_RS05065	1078546..1079103	-	558	WP_025276160.1	hypothetical protein	-
HPOKI102_RS05070	1079104..1079394	-	291	WP_000159826.1	hypothetical protein	-
HPOKI102_RS05075	1079391..1079846	-	456	WP_025276161.1	hypothetical protein	-
HPOKI102_RS05080	1079843..1080784	-	942	WP_025276162.1	Flp pilus assembly complex ATPase component TadA	-
HPOKI102_RS05085	1080784..1081083	-	300	WP_025276163.1	hypothetical protein	-
HPOKI102_RS05090	1081080..1081343	-	264	WP_025276164.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1011592..1108375	96783

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10860.83 Da
• Isoelectric Point: 10.0264

>T45629 WP_015086299.1 NZ_CP006820:1076266-1076547 [Helicobacter pylori oki102]
MLKVRTKKDFLKDFNKHILSGRITESDVASVVDCLKKQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T45629 NZ_CP006820:1076266-1076547 [Helicobacter pylori oki102]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATTTTATCTGGGCGTATTACAGAAAGCGA
TGTTGCAAGCGTTGTTGATTGCCTAAAAAAACAAAAACCACTCCAACAAAAATATTGCGACCATGCTTTGAGTGGCAATC
TTAAAGGGCTGAGAGAGTGTCATGTTAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12212.73 Da
• Isoelectric Point: 5.7657

>AT45629 WP_025276158.1 NZ_CP006820:1075959-1076273 [Helicobacter pylori oki102]
MSNIITDYSQYNEKQLHNFLNSIEKQLLKAEGDKNKAIKKIQECELQERMIRQVLAQKHSQEKEPTQSLLNTIVSKDDPE
CDVSFGDFNDFLQIAKQERERRNA

Nucleotide

• Length: 315 bp

>AT45629 NZ_CP006820:1075959-1076273 [Helicobacter pylori oki102]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACTCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATTCAAGAGTGTGAATTGCAAGAACGAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGTCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCGTAATGCTTAA

Structures

Toxin

• AlphaFold DB: K7Y720

Antitoxin

• AlphaFold DB: X2I3W4