45612

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2207301..2207898 Replicon chromosome
Accession NZ_CP006798
Organism Klebsiella pneumoniae subsp. pneumoniae PittNDM01

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag Q770_RS10815 Protein ID WP_004142563.1
Coordinates 2207581..2207898 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag Q770_RS10810 Protein ID WP_004142561.1
Coordinates 2207301..2207588 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Q770_RS10780 2203381..2203629 + 249 WP_002893037.1 DUF1158 domain-containing protein -
Q770_RS10785 2203647..2203988 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
Q770_RS10790 2204019..2205134 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
Q770_RS10795 2205314..2205895 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
Q770_RS10800 2205895..2206263 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
Q770_RS10805 2206383..2207033 + 651 Protein_2105 oxygen-insensitive NAD(P)H nitroreductase -
Q770_RS10810 2207301..2207588 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
Q770_RS10815 2207581..2207898 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
Q770_RS10820 2208083..2209126 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
Q770_RS10825 2209793..2210659 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
Q770_RS10830 2210768..2212195 + 1428 WP_043906839.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T45612 WP_004142563.1 NZ_CP006798:c2207898-2207581 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T45612 NZ_CP006798:c2207898-2207581 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT45612 WP_004142561.1 NZ_CP006798:c2207588-2207301 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT45612 NZ_CP006798:c2207588-2207301 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References