Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 331909..332495 Replicon chromosome
Accession NZ_CP006722
Organism Klebsiella pneumoniae subsp. pneumoniae 1158

Toxin (Protein)


Gene name doc Uniprot ID A0A7Z9B6M3
Locus tag P243_RS01580 Protein ID WP_029884514.1
Coordinates 332127..332495 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag P243_RS01575 Protein ID WP_004174006.1
Coordinates 331909..332130 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P243_RS01555 328066..328992 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
P243_RS01560 328989..330266 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
P243_RS01565 330263..331030 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
P243_RS01570 331032..331745 + 714 WP_029884513.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
P243_RS01575 331909..332130 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
P243_RS01580 332127..332495 + 369 WP_029884514.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
P243_RS01585 332768..334084 + 1317 WP_029884515.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
P243_RS01590 334191..335078 + 888 WP_004901104.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
P243_RS01595 335075..335920 + 846 WP_004901101.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
P243_RS01600 335922..336992 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 326895..337729 10834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(6-116)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13583.01 Da        Isoelectric Point: 8.6410

>T45455 WP_029884514.1 NZ_CP006722:332127-332495 [Klebsiella pneumoniae subsp. pneumoniae 1158]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRMALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T45455 NZ_CP006722:332127-332495 [Klebsiella pneumoniae subsp. pneumoniae 1158]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCATGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTATGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT45455 WP_004174006.1 NZ_CP006722:331909-332130 [Klebsiella pneumoniae subsp. pneumoniae 1158]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT45455 NZ_CP006722:331909-332130 [Klebsiella pneumoniae subsp. pneumoniae 1158]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z9B6M3

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References