Detailed information of TA system TA045425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1331523..1332090
• Replicon: chromosome
• Accession: NZ_CP006696
• Organism: Xylella fastidiosa subsp. sandyi Ann-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: D934_RS05940
• Protein ID: WP_004085174.1
• Coordinates: 1331809..1332090 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: D934_RS05935
• Protein ID: WP_004085172.1
• Coordinates: 1331523..1331798 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D934_RS05905	1327423..1329297	+	1875	WP_020852856.1	phage major capsid protein	-
D934_RS05910	1329315..1329575	+	261	WP_020852857.1	hypothetical protein	-
D934_RS05915	1329575..1329898	+	324	WP_020852858.1	hypothetical protein	-
D934_RS05920	1329895..1330416	+	522	WP_020850962.1	hypothetical protein	-
D934_RS05925	1330392..1330934	+	543	WP_020850961.1	hypothetical protein	-
D934_RS05930	1330931..1331518	+	588	WP_042836490.1	phage baseplate assembly protein V	-
D934_RS05935	1331523..1331798	-	276	WP_004085172.1	HigA family addiction module antidote protein	Antitoxin
D934_RS05940	1331809..1332090	-	282	WP_004085174.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D934_RS05945	1332288..1332626	+	339	WP_004090694.1	GPW/gp25 family protein	-
D934_RS05950	1332626..1333519	+	894	WP_020852724.1	baseplate J/gp47 family protein	-
D934_RS05955	1333512..1334069	+	558	WP_020852723.1	phage tail protein I	-
D934_RS05960	1334077..1335669	+	1593	WP_020852722.1	tail fiber domain-containing protein	-
D934_RS05965	1335734..1336912	+	1179	WP_020852721.1	phage tail sheath family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1292345..1385055	92710

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10610.17 Da
• Isoelectric Point: 9.8829

>T45425 WP_004085174.1 NZ_CP006696:c1332090-1331809 [Xylella fastidiosa subsp. sandyi Ann-1]
MIKSFRHKGIQQFFLVGSTAGIQTKHAAKLRIQLTALESAKRPEDMNAPGWKLHPLKGADLKGHWSIWVNGNYRLTFAFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T45425 NZ_CP006696:c1332090-1331809 [Xylella fastidiosa subsp. sandyi Ann-1]
ATGATCAAAAGTTTCCGCCACAAAGGCATTCAACAATTTTTCCTTGTGGGATCAACCGCTGGTATCCAAACCAAACACGC
CGCCAAACTGCGTATCCAGCTCACCGCGTTGGAGAGTGCCAAGCGCCCCGAAGATATGAATGCTCCAGGCTGGAAACTGC
ACCCACTAAAAGGCGCTGACTTAAAAGGTCATTGGTCCATCTGGGTGAATGGCAACTATCGCCTCACGTTTGCTTTTGAA
GGCGAAGACGCAATCTTGGTCGACTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10072.68 Da
• Isoelectric Point: 10.2184

>AT45425 WP_004085172.1 NZ_CP006696:c1331798-1331523 [Xylella fastidiosa subsp. sandyi Ann-1]
MKRMHNPAHPGEVLREYLGELTVTNAAAKLGVSRVALSRILNGTNGISADMALRLEDALGTSAEMWAAMQFKYDLWVASQ
QPRPKITRLHV

Nucleotide

• Length: 276 bp

>AT45425 NZ_CP006696:c1331798-1331523 [Xylella fastidiosa subsp. sandyi Ann-1]
ATGAAACGTATGCACAATCCCGCACATCCAGGTGAAGTGTTGCGTGAGTATCTTGGTGAGCTCACTGTGACCAATGCGGC
TGCAAAGCTAGGCGTCAGCCGTGTGGCTTTGTCGCGTATTCTCAATGGTACCAATGGCATCTCTGCCGATATGGCCCTTC
GCCTGGAAGATGCGCTTGGCACCAGTGCAGAGATGTGGGCAGCCATGCAGTTCAAGTATGACCTGTGGGTTGCTAGTCAG
CAGCCACGCCCGAAGATCACGCGCCTTCATGTGTAA