Detailed information of TA system TA045390

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 28832..29489
• Replicon: plasmid pNDM-US
• Accession: NZ_CP006661
• Organism: Klebsiella pneumoniae strain ATCC BAA-2146

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: kpn2146_RS27670
• Protein ID: WP_000270043.1
• Coordinates: 29139..29489 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: kpn2146_RS27665
• Protein ID: WP_000124640.1
• Coordinates: 28832..29134 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
kpn2146_RS27615	24466..24894	+	429	WP_000591076.1	hypothetical protein	-
kpn2146_RS27620	24952..25311	+	360	WP_000422769.1	hypothetical protein	-
kpn2146_RS27625	25311..25757	+	447	WP_000919343.1	hypothetical protein	-
kpn2146_RS27630	25754..26272	+	519	WP_000210757.1	nitrite reductase	-
kpn2146_RS27635	26272..26502	+	231	WP_000972665.1	hypothetical protein	-
kpn2146_RS27640	26489..27346	+	858	WP_001167036.1	hypothetical protein	-
kpn2146_RS33625	27258..27563	+	306	WP_171820680.1	hypothetical protein	-
kpn2146_RS27650	27566..28093	+	528	WP_004201083.1	thermonuclease family protein	-
kpn2146_RS27655	28151..28423	+	273	WP_001043046.1	HU family DNA-binding protein	-
kpn2146_RS27660	28512..28805	+	294	WP_001239998.1	hypothetical protein	-
kpn2146_RS27665	28832..29134	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
kpn2146_RS27670	29139..29489	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
kpn2146_RS27675	29652..30200	+	549	WP_001061573.1	hypothetical protein	-
kpn2146_RS27685	30541..30735	+	195	WP_000343597.1	hypothetical protein	-
kpn2146_RS27690	30746..31117	+	372	WP_000516918.1	hypothetical protein	-
kpn2146_RS27695	31110..31580	+	471	WP_001281821.1	hypothetical protein	-
kpn2146_RS27700	31595..31930	-	336	WP_000683477.1	hypothetical protein	-
kpn2146_RS27705	32027..32515	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
kpn2146_RS27710	32518..33015	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-6 / aac(6')-Ib / qacE / sul1 / rmtC / blaNDM-1	htpB	1..140825	140825

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T45390 WP_000270043.1 NZ_CP006661:c29489-29139 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T45390 NZ_CP006661:c29489-29139 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT45390 WP_000124640.1 NZ_CP006661:c29134-28832 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT45390 NZ_CP006661:c29134-28832 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9