45344

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2072555..2073057 Replicon chromosome
Accession NZ_CP006636
Organism Escherichia coli PCN061

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag PCN061_RS10685 Protein ID WP_000767829.1
Coordinates 2072555..2072809 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag PCN061_RS10690 Protein ID WP_001259255.1
Coordinates 2072806..2073057 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PCN061_RS10670 2069048..2070406 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
PCN061_RS28415 2070396..2070458 - 63 WP_010723108.1 membrane protein YoeI -
PCN061_RS10675 2070673..2071602 - 930 WP_047928782.1 LysR family transcriptional regulator -
PCN061_RS10680 2071648..2072472 - 825 WP_000754737.1 SDR family oxidoreductase -
PCN061_RS10685 2072555..2072809 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
PCN061_RS10690 2072806..2073057 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
PCN061_RS26055 2073341..2073391 + 51 WP_001364200.1 his operon leader peptide -
PCN061_RS10695 2073537..2074436 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
PCN061_RS10700 2074442..2075746 + 1305 WP_001446927.1 histidinol dehydrogenase -
PCN061_RS10705 2075743..2076813 + 1071 WP_047928783.1 histidinol-phosphate transaminase -
PCN061_RS10710 2076813..2077880 + 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2067491..2068699 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T45344 WP_000767829.1 NZ_CP006636:c2072809-2072555 [Escherichia coli PCN061]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T45344 NZ_CP006636:c2072809-2072555 [Escherichia coli PCN061]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT45344 WP_001259255.1 NZ_CP006636:c2073057-2072806 [Escherichia coli PCN061]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT45344 NZ_CP006636:c2073057-2072806 [Escherichia coli PCN061]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References