Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2072555..2073057 Replicon chromosome
Accession NZ_CP006636
Organism Escherichia coli PCN061

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag PCN061_RS10685 Protein ID WP_000767829.1
Coordinates 2072555..2072809 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag PCN061_RS10690 Protein ID WP_001259255.1
Coordinates 2072806..2073057 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PCN061_RS10670 2069048..2070406 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
PCN061_RS28415 2070396..2070458 - 63 WP_010723108.1 membrane protein YoeI -
PCN061_RS10675 2070673..2071602 - 930 WP_047928782.1 LysR family transcriptional regulator -
PCN061_RS10680 2071648..2072472 - 825 WP_000754737.1 SDR family oxidoreductase -
PCN061_RS10685 2072555..2072809 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
PCN061_RS10690 2072806..2073057 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
PCN061_RS26055 2073341..2073391 + 51 WP_001364200.1 his operon leader peptide -
PCN061_RS10695 2073537..2074436 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
PCN061_RS10700 2074442..2075746 + 1305 WP_001446927.1 histidinol dehydrogenase -
PCN061_RS10705 2075743..2076813 + 1071 WP_047928783.1 histidinol-phosphate transaminase -
PCN061_RS10710 2076813..2077880 + 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2067491..2068699 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T45344 WP_000767829.1 NZ_CP006636:c2072809-2072555 [Escherichia coli PCN061]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T45344 NZ_CP006636:c2072809-2072555 [Escherichia coli PCN061]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT45344 WP_001259255.1 NZ_CP006636:c2073057-2072806 [Escherichia coli PCN061]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT45344 NZ_CP006636:c2073057-2072806 [Escherichia coli PCN061]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References