Detailed information of TA system TA045331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 254281..254975
• Replicon: chromosome
• Accession: NZ_CP006636
• Organism: Escherichia coli PCN061

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: PCN061_RS01215
• Protein ID: WP_029403942.1
• Coordinates: 254577..254975 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: PCN061_RS01210
• Protein ID: WP_000554757.1
• Coordinates: 254281..254574 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCN061_RS01190	249921..250418	+	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-
PCN061_RS01195	250634..252346	-	1713	Protein_223	flagellar biosynthesis protein FlhA	-
PCN061_RS01200	252318..253103	+	786	WP_076604657.1	putative lateral flagellar export/assembly protein LafU	-
PCN061_RS01205	253174..254229	+	1056	WP_001226164.1	DNA polymerase IV	-
PCN061_RS01210	254281..254574	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
PCN061_RS01215	254577..254975	+	399	WP_029403942.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
PCN061_RS01220	254985..255437	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
PCN061_RS01225	255683..255886	+	204	Protein_229	RNA ligase RtcB family protein	-
PCN061_RS01230	255885..256406	+	522	Protein_230	peptide chain release factor H	-
PCN061_RS01235	256463..257920	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
PCN061_RS01240	258181..258639	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
PCN061_RS01245	258731..259975	+	1245	WP_000189541.1	esterase FrsA	-
-	259235..259315	+	81	NuclAT_10	-	-
-	259235..259315	+	81	NuclAT_10	-	-
-	259235..259315	+	81	NuclAT_10	-	-
-	259235..259315	+	81	NuclAT_10	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15498.86 Da
• Isoelectric Point: 8.0660

>T45331 WP_029403942.1 NZ_CP006636:254577-254975 [Escherichia coli PCN061]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPYKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T45331 NZ_CP006636:254577-254975 [Escherichia coli PCN061]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTTATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT45331 WP_000554757.1 NZ_CP006636:254281-254574 [Escherichia coli PCN061]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT45331 NZ_CP006636:254281-254574 [Escherichia coli PCN061]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6