Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 248245..248786 Replicon chromosome
Accession NZ_CP006636
Organism Escherichia coli PCN061

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag PCN061_RS01175 Protein ID WP_000615979.1
Coordinates 248245..248523 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag PCN061_RS01180 Protein ID WP_000729704.1
Coordinates 248526..248786 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PCN061_RS01160 245827..246405 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
PCN061_RS01165 246611..247378 + 768 WP_000333379.1 class II glutamine amidotransferase -
PCN061_RS01170 247349..248089 - 741 WP_001225679.1 murein L,D-transpeptidase -
PCN061_RS01175 248245..248523 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
PCN061_RS01180 248526..248786 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
PCN061_RS01185 248972..249745 + 774 WP_001353765.1 C40 family peptidase -
PCN061_RS01190 249921..250418 + 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
PCN061_RS01195 250634..252346 - 1713 Protein_223 flagellar biosynthesis protein FlhA -
PCN061_RS01200 252318..253103 + 786 WP_076604657.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T45330 WP_000615979.1 NZ_CP006636:c248523-248245 [Escherichia coli PCN061]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T45330 NZ_CP006636:c248523-248245 [Escherichia coli PCN061]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT45330 WP_000729704.1 NZ_CP006636:c248786-248526 [Escherichia coli PCN061]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT45330 NZ_CP006636:c248786-248526 [Escherichia coli PCN061]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References