Detailed information of TA system TA045325

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 57743..58169
• Replicon: plasmid p3PCN033
• Accession: NZ_CP006635
• Organism: Escherichia coli PCN033

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: PPECC33_RS24945
• Protein ID: WP_001312861.1
• Coordinates: 57743..57901 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	57945..58169 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPECC33_RS24910	53117..53806	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
PPECC33_RS24915	53993..54376	-	384	WP_001151524.1	relaxosome protein TraM	-
PPECC33_RS24920	54697..55299	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
PPECC33_RS24925	55596..56417	-	822	WP_001234426.1	DUF945 domain-containing protein	-
PPECC33_RS24930	56535..56822	-	288	WP_000107535.1	hypothetical protein	-
PPECC33_RS24945	57743..57901	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	57945..58169	-	225	NuclAT_0	-	Antitoxin
-	57945..58169	-	225	NuclAT_0	-	Antitoxin
-	57945..58169	-	225	NuclAT_0	-	Antitoxin
-	57945..58169	-	225	NuclAT_0	-	Antitoxin
PPECC33_RS28820	57981..58169	+	189	WP_001299721.1	hypothetical protein	-
PPECC33_RS24955	58181..58900	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
PPECC33_RS24960	58897..59331	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
PPECC33_RS27265	59386..59583	-	198	Protein_74	hypothetical protein	-
PPECC33_RS24965	59611..59844	-	234	WP_000005987.1	DUF905 family protein	-
PPECC33_RS24970	59912..60451	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
PPECC33_RS29015	60477..60683	-	207	WP_000275856.1	hypothetical protein	-
PPECC33_RS24985	61822..62793	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / sitABCD / oqxA / oqxB / blaTEM-1B / tet(B)	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN	1..161511	161511

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T45325 WP_001312861.1 NZ_CP006635:c57901-57743 [Escherichia coli PCN033]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T45325 NZ_CP006635:c57901-57743 [Escherichia coli PCN033]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT45325 NZ_CP006635:c58169-57945 [Escherichia coli PCN033]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin