Detailed information of TA system TA045320

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19414..19668
• Replicon: plasmid p3PCN033
• Accession: NZ_CP006635
• Organism: Escherichia coli PCN033

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: PPECC33_RS24720
• Protein ID: WP_001351576.1
• Coordinates: 19414..19620 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	19607..19668 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPECC33_RS24685	14533..15348	-	816	WP_001043265.1	sulfonamide-resistant dihydropteroate synthase Sul2	-
PPECC33_RS24690	15656..16507	-	852	WP_000240536.1	replication protein	-
PPECC33_RS24695	16494..17213	-	720	Protein_21	AAA family ATPase	-
PPECC33_RS24700	17263..17967	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
PPECC33_RS24705	18092..18574	-	483	WP_001273588.1	hypothetical protein	-
PPECC33_RS24710	18567..18641	-	75	WP_001442103.1	RepA leader peptide Tap	-
PPECC33_RS24715	18873..19130	-	258	WP_000083845.1	replication regulatory protein RepA	-
PPECC33_RS24720	19414..19620	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	19607..19668	+	62	NuclAT_1	-	Antitoxin
-	19607..19668	+	62	NuclAT_1	-	Antitoxin
-	19607..19668	+	62	NuclAT_1	-	Antitoxin
-	19607..19668	+	62	NuclAT_1	-	Antitoxin
PPECC33_RS29010	19924..19998	-	75	Protein_27	endonuclease	-
PPECC33_RS24725	20244..20456	-	213	WP_001299730.1	ANR family transcriptional regulator	-
PPECC33_RS24730	20592..21152	-	561	WP_000139315.1	fertility inhibition protein FinO	-
PPECC33_RS24735	21255..22115	-	861	WP_000704514.1	alpha/beta hydrolase	-
PPECC33_RS24740	22174..22920	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / sitABCD / oqxA / oqxB / blaTEM-1B / tet(B)	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN	1..161511	161511

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T45320 WP_001351576.1 NZ_CP006635:c19620-19414 [Escherichia coli PCN033]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T45320 NZ_CP006635:c19620-19414 [Escherichia coli PCN033]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT45320 NZ_CP006635:19607-19668 [Escherichia coli PCN033]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin