Detailed information of TA system TA045313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3497595..3498394
• Replicon: chromosome
• Accession: NZ_CP006632
• Organism: Escherichia coli PCN033

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: PPECC33_RS17195
• Protein ID: WP_000347273.1
• Coordinates: 3497930..3498394 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: PPECC33_RS17190
• Protein ID: WP_001307405.1
• Coordinates: 3497595..3497930 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPECC33_RS17175	3493380..3494150	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
PPECC33_RS17180	3494166..3495500	-	1335	WP_000599654.1	galactarate/glucarate/glycerate transporter GarP	-
PPECC33_RS17185	3495875..3497446	+	1572	WP_001273756.1	galactarate dehydratase	-
PPECC33_RS17190	3497595..3497930	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
PPECC33_RS17195	3497930..3498394	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
PPECC33_RS17200	3498449..3499258	-	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
PPECC33_RS17205	3499507..3500787	+	1281	WP_000681942.1	tagatose-bisphosphate aldolase subunit KbaZ	-
PPECC33_RS17210	3500810..3501283	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
PPECC33_RS17215	3501294..3502073	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
PPECC33_RS17220	3502063..3502941	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
PPECC33_RS17225	3502959..3503393	+	435	WP_000948814.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3488955..3498394	9439

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T45313 WP_000347273.1 NZ_CP006632:3497930-3498394 [Escherichia coli PCN033]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T45313 NZ_CP006632:3497930-3498394 [Escherichia coli PCN033]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT45313 WP_001307405.1 NZ_CP006632:3497595-3497930 [Escherichia coli PCN033]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT45313 NZ_CP006632:3497595-3497930 [Escherichia coli PCN033]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: A0A0E0XVC7