Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2196547..2197049 Replicon chromosome
Accession NZ_CP006632
Organism Escherichia coli PCN033

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag PPECC33_RS10875 Protein ID WP_000767829.1
Coordinates 2196547..2196801 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A6N8RC01
Locus tag PPECC33_RS10880 Protein ID WP_001259251.1
Coordinates 2196798..2197049 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPECC33_RS10860 2193040..2194398 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
PPECC33_RS28220 2194388..2194450 - 63 WP_010723108.1 membrane protein YoeI -
PPECC33_RS10865 2194665..2195594 - 930 WP_000803351.1 LysR family transcriptional regulator -
PPECC33_RS10870 2195640..2196464 - 825 WP_000754739.1 SDR family oxidoreductase -
PPECC33_RS10875 2196547..2196801 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
PPECC33_RS10880 2196798..2197049 - 252 WP_001259251.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
PPECC33_RS26340 2197333..2197383 + 51 WP_001364200.1 his operon leader peptide -
PPECC33_RS10885 2197529..2198428 + 900 WP_000131790.1 ATP phosphoribosyltransferase -
PPECC33_RS10890 2198434..2199738 + 1305 WP_000009584.1 histidinol dehydrogenase -
PPECC33_RS10895 2199735..2200805 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
PPECC33_RS10900 2200805..2201872 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T45311 WP_000767829.1 NZ_CP006632:c2196801-2196547 [Escherichia coli PCN033]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T45311 NZ_CP006632:c2196801-2196547 [Escherichia coli PCN033]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9293.56 Da        Isoelectric Point: 4.7815

>AT45311 WP_001259251.1 NZ_CP006632:c2197049-2196798 [Escherichia coli PCN033]
MRTISYSEARQNLSATMMKAVDDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT45311 NZ_CP006632:c2197049-2196798 [Escherichia coli PCN033]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGATGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAGGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCTGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A6N8RC01

References