Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 327527..328221 Replicon chromosome
Accession NZ_CP006632
Organism Escherichia coli PCN033

Toxin (Protein)


Gene name yafO Uniprot ID A0A1X0YXS1
Locus tag PPECC33_RS01555 Protein ID WP_001263484.1
Coordinates 327823..328221 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag PPECC33_RS01550 Protein ID WP_000554755.1
Coordinates 327527..327820 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPECC33_RS01525 323344..323811 + 468 WP_000725257.1 flagellar basal body-associated FliL family protein -
PPECC33_RS01530 323831..324547 + 717 WP_000938719.1 FliA/WhiG family RNA polymerase sigma factor -
PPECC33_RS01535 324560..325423 + 864 WP_001169519.1 flagellar motor stator protein MotA -
PPECC33_RS01540 325426..326349 + 924 WP_001232557.1 putative lateral flagellar export/assembly protein LafU -
PPECC33_RS01545 326420..327475 + 1056 WP_001226183.1 DNA polymerase IV -
PPECC33_RS01550 327527..327820 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
PPECC33_RS01555 327823..328221 + 399 WP_001263484.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
PPECC33_RS01560 328231..328683 + 453 WP_001059899.1 GNAT family N-acetyltransferase -
PPECC33_RS01565 328928..329134 + 207 Protein_298 RtcB family protein -
PPECC33_RS01570 329130..329482 + 353 Protein_299 peptide chain release factor H -
PPECC33_RS01575 329539..330996 - 1458 WP_001293004.1 cytosol nonspecific dipeptidase -
PPECC33_RS01580 331257..331715 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
PPECC33_RS01585 331807..333051 + 1245 WP_000189541.1 esterase FrsA -
- 332311..332391 + 81 NuclAT_7 - -
- 332311..332391 + 81 NuclAT_7 - -
- 332311..332391 + 81 NuclAT_7 - -
- 332311..332391 + 81 NuclAT_7 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15404.81 Da        Isoelectric Point: 7.3840

>T45296 WP_001263484.1 NZ_CP006632:327823-328221 [Escherichia coli PCN033]
MRVFKTKLICLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T45296 NZ_CP006632:327823-328221 [Escherichia coli PCN033]
ATGCGGGTATTCAAAACAAAACTTATTTGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT45296 WP_000554755.1 NZ_CP006632:327527-327820 [Escherichia coli PCN033]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT45296 NZ_CP006632:327527-327820 [Escherichia coli PCN033]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1X0YXS1

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References